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The objective of this study was to determine the acute toxicity of MEAHA-Fe to the earthworm Eisenia foetida (Savigny 1826). A range-finding test was carried out to determine the concentrations of the test substance that caused 100% and 0% mortality. According to the results obtained in the range-finding test, a definitive test was carried out with the following treatments: 0 (water control) and 1000 mg/kg of artificial soil. Forty earthworms per treatment were used in the definitive test, distributed into four replicates of 10 earthworms each. The test was carried out in polyethylene containers placed inside a room with continuous light for fourteen days. Mortality was determined at 7 and 14 days of incubation. Temperature and humidity were both measured throughout the test and were within acceptable limits. The control treatment did not cause any mortality to the earthworms during 14 days exposure period.
The highest tested concentration (1000 mg/kg of artificial soil) of test substance did not cause mortality to the test organisms. Therefore, the 14-days LC50 value, of the acute toxicity test, for earthworm E. foetida exposed to tested substance was considered greater than 1000 mg/kg of artificial soil.
In a standard OECD 207 test, the 14-days LC50, of the acute toxicity test, for earthworm Eisenia foetida exposed to test substance MEAHA-Fe was considered higher than 1000 mg/kg of artificial soil.
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