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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The NOEC of the test item is 1000 mg/L. The test substance caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit concentration of 1000 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 (2010) was carried out with the test item with the definitive exposure on 2012-11-29 atDr.U.Noack-Laboratorien, 31157 Sarstedt, Germany.Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the limit concentration 1000 mg/L pH adjusted. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The inhibition of respiration for the test item replicates ranged from 0 to 9%.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 91.4 mg/L.

The validity criteria of the test guideline were fulfilled.

NOEC and EC-Values of the test substance





> 1000


> 1000


> 1000

                        *) No statistically significant effect (p 0.05), ANOVA