Lithium 2-aminobenzothiazole-6-sulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 January - 15 May 2019.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004.

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Lithium 2-aminobenzothiazole-6-sulphonate

EC Number:

265-367-3

EC Name:

Lithium 2-aminobenzothiazole-6-sulphonate

Cas Number:

65072-36-8

Molecular formula:

C7H6N2O3S2.Li

IUPAC Name:

lithium 2-amino-1,3-benzothiazole-6-sulfonate

Test material form:

solid: particulate/powder

Details on test material:

- Bulk Density (g/ml): 0.39
- Particle size distribution (μm): 19.6
- Water solubility: 20%

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: IR2510181
- Expiration date of the lot/batch: 25 October 2019
- Purity test date: Not specified

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature
- Stability under test conditions: Not specified
- Solubility and stability of the test substance in the solvent/vehicle: soluble at 100 mg/L in dilution water (ISO medium)

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in ISO medium
- Preliminary purification step (if any): Not specified
- Final dilution of a dissolved solid, stock liquid or gel: 100 mg/L (nominal)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L.
- Analytical measurements were performed at the start and end of the test.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation (mean and range, SD): < 24 hours.
- Source: István Szent University, 2100 Gödöllő, Páter Károly u. 1, Hungary
- Feeding during test: No.

ACCLIMATION
- Acclimation period: There was no acclimatization because the water used was similar to the culture water.

Study design

Test type:

static

Water media type:

other: ISO medium, reconstituted water

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

The reconstituted water (ISO medium) had a total hardness of 245 mg/L (as CaCO3).

Test temperature:

The water temperature was measured at the start of the experiment and in 24-hour intervals thereafter in each test vessel. The test temperature was in the range of 20.0 – 20.3°C measured in the test vessels.

pH:

The pH of the test solution was not adjusted and not varied by more than 1.5 units in any one test. The pH was measured at the start and at the end of the test in each test vessel and was in the range of 7.81 – 7.94.

Dissolved oxygen:

The dissolved oxygen concentration was measured in each test vessel at the start and at the end of the test and was in the range of 8.5 – 8.7 mg/L.

Salinity:

Not applicable.

Nominal and measured concentrations:

Nominal concentration: 100 mg/L.
Measured concentration (start of study): 95.6 ± 0.567 mg/L
Measured concentration (end of study): 96.8 ± 1.486 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Type: Closed
- Volume of solution: At least 5 mL test solution/animal.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): Not applicable.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Details on results:

One animal (out of 20) was observed to be immobilised at the end of the 48-hour test period. No treated animals were immobilised after 24 hours, and no control animals were immobilised at any point during the study.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute immobilisation test in Daphnia magna, lithium 2-aminobenzothiazole-6-sulphonate was found to have an EC50 > 100 mg/L.

Executive summary:

In an OECD guideline study, conducted to GLP, lithium 2-aminobenzothiazole-6-sulphonate was found to have an EC50 > 100 mg/L in Daphnia magna. One treated animal (out of 20) was found to be immobilised at the end of the 48-hour study.