Registration Dossier
Registration Dossier
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EC number: 617-694-1 | CAS number: 85261-19-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- EC Number:
- 617-694-1
- Cas Number:
- 85261-19-4
- Molecular formula:
- C16H33NO6
- IUPAC Name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- Test material form:
- solid
- Details on test material:
- sample no. MTDID 48740
MEGA 9: 98.1 % (GC)
Water content: 0.3%
pH (aqueous solution 1%): 7.5
Expiry Date: 2017-12
Product Nr: MTDID 48740
Batch: 549612
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Algae was obtainend from canadian Phycological Culture Centre.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- pH:
- 7.7-7.9
- Nominal and measured concentrations:
- nominal 6.25 - 12.5 - 25 - 50 - 100 mg/L and control
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All EC10 and EC50 values was > 100 mg/L. NOEC and LOEC was found to be 100 mg/L.
- Executive summary:
A samp le of MTDID 48740 was tested to assess its pot ent ial to cause effects on the
growth of the green algae, Pseudokirchneriel/a subcapitata, using t he procedures
out lined in the Maxxam Study Plan for TOX1713, which is based on the Standard
Operating Procedure {SOP) for OECD 201: Alga, Growth Inhibit ion (BBY2SOP-00016) and
OECD 201 Freshwater Alga and Cyanobacteria, Growt h Inhibit ion Test (OECD, 2011).
This test was conducted to assess the effects of the test item on the algae by observing
the growth of test organisms over a 72-h exposure period, under cont rolled condit ions,
using the following nominal concentrations: 6.25, 12.5, 25, 50, and 100 mg Test lt em/ L
in addi t ion to a negative (laboratory) cont rol {0 mg/L).
The mean measured concent rat ions of the test item were wit hin 80% to 120% {93% to
103%) of the nominal concentrations; therefore, the biological endpoints were
calculated using the nominal concentrations of the test item. The 72-h EC50, EC20, and
EC10 with 95% Confidence Limits and NOEC/LOEC for cell yield and specific growt h rate
are summarised in the table below, based on the nominal concentrat ion of MTDID
48740.
Summary of the Test Endpoints Values {Nominal Concentration)
Measurement Type Cell Yield Specific Growth Rate
72-h ErC50 > 100 mg/L
72-h EyC50 > 100 mg/L
NOEC/LOEC (mg/L)100/N/A
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