Dodecamethylenediamine

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05. Nov. 2019 - 13. Jan. 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: B1-5-10
- Expiration date of the lot/batch: 31. Jan. 2020
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL

- Storage condition of test material: The test item was stored in a closed vessel at room temperature (20 ± 5°C), dry, in the
dark.
- Stability under storage conditions: assumed stable, no indication to the contrary
- Stability under test conditions: assumed stable, assumed stable
- Solubility and stability of the test substance in the solvent/dispersant/vehicle/test medium: tp be tested
- Reactivity of the test substance with the solvent/vehicle /test medium (if applicable): not applicable

Key result

Water solubility:

0.36 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

2 g/L

Incubation duration:

3 d

Temp.:

19.5 °C

pH:

ca. 11

Key result

Water solubility:

0.52 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

20 g/L

Incubation duration:

3 d

Temp.:

19.5 °C

pH:

ca. 11

Details on results:

As the solubility was dependent on the nominal concentration, no exact value for the solu-
bility of the test item in demineralised water can be stated. The solubility range for the
tested nominal concentration 2 – 20 g/L is 0.36 – 0.52 g/L at 19.5 °C, corresponding to
0.36 – 0.52 kg/m3 in SI units.

Conclusions:

For a tested nominal range of 2 – 20 g/L, the solubility of the test item in
demineralised water was determined as
0.36 – 0.52 g/L at 19.5 °C

Executive summary:

The solubility of the test item 1,12-Diaminododecane in water using the flask method was

determined using a validated method for LC-MS-MS measurements.

The preliminary test was performed during validation and showed that the solubility of the

test item is dependent on the weighed amount. Thus, six different loading rates were cho-

sen for the main test: 2 / 3.5 / 5 / 11 / 17 / 20 g/L (nominal). The three flasks 1A-C with the

same loading rate (11 g/L) were used during equilibration.

Seven vessels (flasks blank, 1C (for the sampling point 72 h) and 2 – 6) were set onto the

shaking apparatus immediately. After 24 ± 2 h, flask 1B (for the sampling point 48 h) and

after 48 ± 2 h, flask 1A (for the sampling point 24 h) were set onto the shaking apparatus

and all flasks were shaken for further 24 ± 2 h at room temperature. Then, flasks 1A-C

were taken from the shaking apparatus, samples were taken, membrane filtrated and ana-

lysed using LC-MS-MS. Because of a difference of less than 15 % in the concentrations on

days 2 and 3 and no upward tendency in the last three values, the test was finished.

The final measurement was performed as determination from flasks 2 – 6. The pH value in

the flasks 2 – 6 lay in the range of 11.10 – 11.22.

No Tyndall effect (i.e. a laser beam was not scattered when transmitted through the liquid)

could be observed indicating that no colloidal dispersed particles were present.

Dependency of solubility on amount of the test item (nominal load) was perceived in the

main study, too. For a tested nominal range of 2 – 20 g/L, the solubility of the test item in

demineralised water was determined as

0.36 – 0.52 g/L at 19.5 °C  

= 0.36 – 0.52 kg/m3 in SI units

Description of key information

For a tested nominal range of 2 – 20 g/L, the solubility of the test item in

demineralised water was determined as

0.36 – 0.52 g/L at 19.5 °C

Key value for chemical safety assessment

Water solubility:

0.52 g/L

at the temperature of:

19.5 °C