Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report Date:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Name 1,2,4,5-Tetramethylimidazole
Batch no. EV7YH
Appearance white crystalline powder
Composition 1,2,4,5-Tetramethylimidazol: ≥98%
CAS No. 1739-83-9
EC-No. 217-100-7
Molecular formula C7H12N2
Molecular weight 124.187 g/mol

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
ca. 28 d

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
ca. 9
Sampling time:
28 d

Any other information on results incl. tables

The test item1,2,4,5-Tetramethylimidazolewas tested using a concentration of nominally 20 mg organic carbon/L1,2,4,5-Tetramethylimidazolein test medium following OECD 301B and EU-Method C.4-C.

 

Aniline was chosen as positive control.

Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.

All validity criteria were met. Degradation of the positive control surpassed the pass level of 60 % after 7 days.

 

The following data were determined for the test item1,2,4,5-Tetramethylimidazole:

10-day-window:                                                                                                             not detected
degradation at the end of 10-day-window                                                                             none
degradation at the end of the test                                                                                              9 %

pass level following guideline:      60 % at the end of 10-day-window for pure substances

                                                                        respective 60 % at the end of the test for mixtures

 

Therefore, when applying the 10-day-window, the test item1,2,4,5-Tetramethylimidazoleisnotreadily biodegradablefollowing OECD 301B and EU C.4-C respectively. As degradation missed the pass level of 60% in the course of the test,1,2,4,5-Tetramethylimidazoleis considered asnotultimately biodegradable,after 28 days, as well.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable