Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04.2018 to 05.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: SEPA (HJ/T 153-2004), The guidelines for testing of chemicals

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

(1992)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: Chemicals - Ready biodegradability - Manometric respirometry test (GB/T21801-2008)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated

EC Number:

810-418-2

Cas Number:

1703784-30-8

IUPAC Name:

Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated

Test material form:

liquid

Specific details on test material used for the study:

Lot number: 890000410422
Expiration date: 2018-08-02
Purity: 100%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): A fresh sample of activated sludge from a domesticwast water treatment plant (Nanjing Chengdong Sewage Treatment Plant) was collected. (Batch No.: AS201804101)
- Laboratory culture: no
- Preparation of inoculum for exposure: The sludge was 3 times washed with basal mineral salt medium and aerated until use. Before use, the sludge was centrifuged one time (5000 r/min, 4°C, 10 minutes). Thereafter a small amount of the washed sludge was weighed and then dried at 100°C until the weight of the dried sludge did not change significantly anymore. Then the dry weight percentage of suspended solids (p) of the activated sludge was calculated to be 9.11%. Finally 22.0 g wet sludge after centrifugation was mixed with 0.5 L BSM to obtain an activated sludge with a mixed liquor suspended solids level of 4 g/L.

Amount of suspended solids: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: For the preparation of the stock solutions for mineral medium the following reagents (analytical grade) were used:
( a ) KH2PO4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 x 2 H2O 33.40 g/L
NH4Cl 0.50 g/L
pH 7.34 ± 0.2

( b ) CaCl2 x 2 H2O 27.50 g/L
( c ) MgSO4 x 7 H2O 22.50 g/L
( d ) FeCl3 x 6 H2O 0.25 g/L.
The mineral medium applied in the test contained 10 mL/L of mineral stock solution a and 1 mL/L of the mineral stock solution b–d, respectively.

- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 +/- 1°C
- pH: 6.40 ~ 7.36
- Aeration of dilution water: not reported
- Suspended solids concentration: 30 mg dry mass / L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: respirometer
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: activated sludge
- Measuring equipment: respirometer: Automatic respirometer (BOD meter)
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: suitable absorbent used


SAMPLING
- Sampling method: oxygen uptake was measured
- Sample storage before analysis: no

CONTROL AND BLANK SYSTEM
- Inoculum blank: performed
- Toxicity control: performed
- Other: procedural control with reference substance performed

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

6.8

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance anilin was biodegraded by 74% within 14 d thus fulfilling the guideline criterion.

Any other information on results incl. tables

The biodegradation of Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated in the static test was found to be at mean 6.8% for a concentration of 50 mg test item per liter after 28 days. With degradation rates below 10 % over the complete test duration no degradation rate within a 10-day-window can be calculated.

The degradation of the reference substance sodium benzoate had reached 74% within the first 14 days. The difference of extremes of replicate values of the removal of the test item at the end of the test is less than 20%. Therefore, the test can be considered as valid. No inhibitory effects of the test item were observed (more than 25% degradation occurred within 14 days) in the toxicity control.

Conclusion:

According to the guideline, Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated must be considered as being not readily biodegradable under the test conditions applied.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

According to the guideline, PU-2017-793 must be considered as being not readily biodegradable under the test conditions applied.

Executive summary:

The ready biodegradation of Neodecanoic acid, tin (2+) salt (2:1) was investigated in a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) and EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test) over a period of 28 days and using non-adapted activated sludge as inoculum. The biodegradation rate was determined by measurement of oxygen consumption. Inoculum blank, procedural/functional control with the reference substance sodium benzoate, and toxicity control using 100 mg/L test item and 100 mg/L reference compound were performed.

The biodegradation of Neodecanoic acid, tin (2+) salt (2:1) in the static test was found to be at mean 1% with a standard deviation of 1.1% for a concentration of 100 mg test item per liter and at mean 12% with a standard deviation of 9.2% for a concentration of 32 mg test item per liter after 28 days. However, with 14 % (SD = 9.2 %), the maximum mean degradation rate within the 28 day test duration was found in the 32 mg/L assays. For a concentration of 32 mg test item per liter, biodegradation within the 10-day-window was found to be 13%. The 10-day-window started at mean at day 15. With degradation rates below 10 % over the complete test duration in the 100 mg/L assays, no degradation rate within a 10-day-window can be calculated.

The degradation of the reference substance sodium benzoate had reached 90% within the first 14 days. The difference of extremes of replicate values of the removal of the test item at the end of the test for 100 mg/L and 32 mg/L, respectively, is less than 20%. Therefore, the test can be considered as valid. No inhibitory effects of the test item were observed (more than 25% degradation occurred within 14 days) in the toxicity control.

Conclusion:

According to the guideline, Neodecanoic acid, tin (2+) salt (2:1) must be considered as being not readily biodegradable under the test conditions applied.