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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1978
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
only 2 strains tested
Principles of method if other than guideline:
The mutagenic assay used was that of Ames, which employs histidine-requiring auxotrophs of S. typhimurium. S. typhimurium strains TA 1535 and TA 100
were used to detect base substitution mutations; frame shift mutations were determined with strains TA 1538 and TA 98.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

1
Chemical structure
Reference substance name:
2-amino-4,6-dinitrophenol
EC Number:
202-544-6
EC Name:
2-amino-4,6-dinitrophenol
Cas Number:
96-91-3
Molecular formula:
C6H5N3O5
IUPAC Name:
2-amino-4,6-dinitrophenol

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
For metabolic activation studies, reduced nicotinamide adenine dinucleotide phosphate-dependent microsomal enzymes in a rat liver homogenate were
added to the system.
Test concentrations with justification for top dose:
1, 10, 20, 50, 100, 200 mcg/plate

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified

Applicant's summary and conclusion

Conclusions:
Picramic acid at the concentration 1 mcg/plate induced both base pair substitution and frame shift-type mutations in histidine-requiring strains of Salmonella typhimurium without activation by rat liver preparation.
Executive summary:

A similar to OECD 471 test with picramic acid was performed. At the concentration 1 mcg/plate induced both base pair substitution  and  frame shift-type mutations in histidine-requiring strains of Salmonella typhimurium without activation by rat liver preparation.