(2E,2'E)-2,4,8,10-tetraoxaspiro[5.5]undecane-3,9-diylbis(2-methylpropane-2,1-diyl) bis(2-cyano-3-(3,4-dimethoxyphenyl)acrylate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2E,2'E)-2,4,8,10-tetraoxaspiro[5.5]undecane-3,9-diylbis(2-methylpropane-2,1-diyl) bis(2-cyano-3-(3,4-dimethoxyphenyl)acrylate)

Cas Number:

2233585-18-5

Molecular formula:

C39H46N2O12

IUPAC Name:

(2E,2'E)-2,4,8,10-tetraoxaspiro[5.5]undecane-3,9-diylbis(2-methylpropane-2,1-diyl) bis(2-cyano-3-(3,4-dimethoxyphenyl)acrylate)

Test material form:

solid

Remarks:

Light yellow solid (powder)

Specific details on test material used for the study:

Young Daphnia magna ≤ 24 hours old were obtained from the culture kept at the Ecotoxicology Laboratory, maintenance and cultivation records are archived at BIOAGRI.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test system Daphnia magna was obtained from the CAROLINA BIOLOGICAL SUPPLY COMPANY - Burlington, North Carolina (USA) and maintained as a stock culture at BIOAGRI.

Study design

Test type:

static

Water media type:

other: dilution water

Total exposure duration:

48 h

Test conditions

Hardness:

236.2 mg.L-1 of CaCO3

Test temperature:

20.2 to 20.4°C

pH:

7.7

Dissolved oxygen:

8.1 mg O2/L

Details on test conditions:

In the range-finding test the exposure conditions were:

a) Total number of organisms per each tested concentration or control: 10;
b) Number of replicates per each tested concentration and control: 2;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 0.01; 0.1; 1; 10 and 100 mg.L-1;
g) Temperature of the test solutions: 20.1 to 20.3°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks.

In the definitive test the exposure conditions were:

a) Total number of organisms per each tested concentration or control: 20;
b) Number of replicates per each tested concentration and control: 4;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 6.25; 12.5; 25; 50 and 100 mg.L-1;
g) Temperature of the test solutions: 20.2 to 20.4°C;
h) Photoperiod: 16 hours light and 8 hours dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks;
j) Analytical determination: content dissolved oxygen, pH of the test solutions and the test item concentration were determined at the beginning of the test and after 48 hours.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7) was used as the reference item in order to evaluate the sensitivity of the test organisms. The following test concentrations were used: control, 0.32, 0.56, 1.0, 1.8 and 2.4 mg.L-1.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 91.7 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 51.76 mg/L

Details on results:

In the definitive test the highest tested concentration that did not cause acute effect (immobility) to the organisms after 48 hours was 25 mg.L-1. The lowest concentration that caused 100% immobility was 100 mg.L-1.

Results with reference substance (positive control):

The 24-hours EC50 value of the reference item (potassium dichromate) was 0.84 mg.L-1 (95% confidence limits = 0.71 to 0.99 mg.L-1), showing the sensitivity of the test system (ISO 6341 / OECD 202, 2004).

Any other information on results incl. tables

The test was valid to taking care of the following conditions:

 

·  In the control it was not exceed 10 per cent of immobilized on organism;

·  The dissolved oxygen concentration at the end of the test was ≥ 3 mg.L^{-inthe control and the concentrations test.}

 

Note:For the first item more then 10 per cent of daphnia did not show an immobilized in the control or others sings of disease or stress, for example, discoloration or unusual behavior such as trapping at surface of water.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hours EC50 value of Chiguard GA403 was 51.76 mg.L-1 (95% confidence limits = 44.37 to 60.39 mg.L-1).