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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 12 August 2019 to 6 September 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
- Common name: Pseudokirchneriella subcapitata
- Strain: 278/4
- Source (laboratory, culture collection): SAMS Limited
- Method of cultivation: Freshwater with nutrient medium (3N=BBM+V) was autoclaved at 120 ºC for 15 minutes. Pre-cultures in the exponential growth phase were then prepared from stock laboratory cultures by inoculating the freshwater 2 to 3 days before test commencement.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
21 – 24 ºC 2 °C
pH:
7.5 ±0.1
Nominal and measured concentrations:
Nominal concentrations: 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL capacity borosilicate glass crystallising dishes
- Type: closed
- Volume of solution: 50 mL
- Aeration: No aeration during the 72 hour study
- Type of flow-through: static
- Initial cells density: initial cell density of approximately 494.5 x 10^4 cells per millilitre
- No. of vessels per concentration (replicates): three
- No. of vessels per control (replicates): six

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Light intensity and quality: 4440 to 8880 lx

TEST CONCENTRATIONS
- Test concentrations: 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L (definitive test)
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 1 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- Exponential growth in the control (for algal test): yes
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- 72h EC50: 1.503 mg/L
Reported statistics and error estimates:
The result is based on nominal concentrations and was calculated by non-linear regression within the CETIS suite of statistical analysis
Validity criteria fulfilled:
yes
Conclusions:
The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.
Executive summary:

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to algae was determined during a GLP-compliant

study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Description of key information

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to algae was determined during a GLP-compliant

study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Key value for chemical safety assessment

EC50 for freshwater algae:
10 g/L
EC10 or NOEC for freshwater algae:
1 000 mg/L

Additional information