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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
The experimental start date was 03 Aug 2018, and the experimental completion date was 15 Aug 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Version / remarks:
2011
GLP compliance:
yes
Specific details on test material used for the study:
Purity: UVCB; solid matter 41.4%
Analytical monitoring:
yes
Details on sampling:
- Concentrations: the control and the WAF at 100 mg solid matter/L
- Sampling method: At the end of the exposure period, the replicates with algae were pooled at each concentration before sampling
- Sample storage conditions before analysis: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fraction (WAF) loading rate of 100 mg solid matter/L
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: NIVA CHL 1
- Source: In-house laboratory culture
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Hardness:
24 mg CaCO3/L
Test temperature:
22 - 23 °C
pH:
8.0 - 8.4
Nominal and measured concentrations:
Nominal: 100 mg solid matter/L
Measured: 54 mg solid matter/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL, all-glass with aluminium caps
- Type: closed
- Aeration: none
- Initial cells density: 1 x 10E4 cells/mL
- Control end cells density: 283.7 x 10E4 cells/mL
- No. of vessels per concentration: 6
- No. of vessels per control : 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: Continuously using TLD-lamps with a light intensity within the range of 85 to 86 μE.m-2.s-1

EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: At the beginning of the test, cells were counted using a microscope and a counting chamber.Thereafter, cell densities were determined by spectrophotometric measurement of samples at 680 nm using a spectrophotometer with immersion probe (path length = 10 mm); every 24-hours
- Other: At the end of the test microscopic observations were performed on the WAF at 100 mg solid matter/L and the control to observe for any abnormal appearance of the algae

TEST CONCENTRATIONS
combined limit/range-finding test maximum concentration 100 mg solid matter/L
Reference substance (positive control):
yes
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
< 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
< 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
- Exponential growth in the control: yes
- Observation of abnormalities none
- Unusual cell shape: normal and healthy appearance
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- EC50: The EC50 for growth rate inhibition (72h-ERC50) was 0.90 mg/L
- Other: Within historical range
Validity criteria fulfilled:
yes
Conclusions:
The 72h-EL50 for inhibition of both growth rate (ERL50) and yield (EYL50) was >100 mg solid matter/L.

The 72h-NOELR for growth rate inhibition was <100 mg solid matter/L when based on statistical analysis and 100 mg solid matter/L when based on biological relevance.

The 72h-NOELR for yield inhibition was <100 mg solid matter/L when based on both statistical analysis and biological relevance.

The analytically confirmed average concentration of dioctyl phosphate in the test solution was 54 mg/L.
Executive summary:

The toxicity to algae of the test item was assessed following OECD 201. During a combined range-finding/limit test study, a study was performed with a maximum WAF of the test item at 100 mg solid matter/L. Microscopic observations at the end of the test revealed a normal and healthy appearance of the algal cells exposed to the WAF at 100 mg solid matter/L when compared to the control.  The 72h-EL50 for inhibition of both growth rate (ErL50) and yield (EyL50) was >100 mg solid matter/L. The 72h-NOELR for growth rate inhibition was <100 mg solid matter/L when based on statistical analysis and 100 mg solid matter/L when based on biological relevance. The 72h-NOELR for yield inhibition was <100 mg solid matter/L when based on both statistical analysis and biological relevance. The analytically confirmed average concentration of dioctyl phosphate in the test solution was 54 mg/L.

Description of key information

The toxicity to algae of the test item was assessed following OECD 201. During a combined range-finding/limit test study, a study was performed with a maximum WAF of the test item at 100 mg solid matter/L. Microscopic observations at the end of the test revealed a normal and healthy appearance of the algal cells exposed to the WAF at 100 mg solid matter/L when compared to the control.  The 72h-EL50 for inhibition of both growth rate (ErL50) and yield (EyL50) was >100 mg solid matter/L. The 72h-NOELR for growth rate inhibition was <100 mg solid matter/L when based on statistical analysis and 100 mg solid matter/L when based on biological relevance. The 72h-NOELR for yield inhibition was <100 mg solid matter/L when based on both statistical analysis and biological relevance. The analytically confirmed average concentration of dioctyl phosphate in the test solution was 54 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information