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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
The experimental start date was 03 Aug 2018, and the experimental completion date was 15 Aug 2018.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
GLP compliance:
yes
Specific details on test material used for the study:
UVCB; solid matter 41.4%
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg solid matter/L
- Sampling method: At the end of the exposure period, the replicates were pooled at each concentration before sampling.
- Sample storage conditions before analysis: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fraction (WAF) loading rate of 100 mg solid matter/L
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus, 1820
- Age at study initiation: less than 24 hours old, from parental daphnids of more than two weeks old
- Source: In-house laboratory culture with a known history
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
180 mg/L expressed as CaCO3
Test temperature:
20 to 21°C
pH:
7.9
Dissolved oxygen:
8.2 to 8.6 mg/L
Nominal and measured concentrations:
Loading: 100 mg solid matter/L
Measured: 16 mg solid matter/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 60 mL, all-glass
- Type: open
- Aeration: None
- No. of organisms per vessel: 5 per vessel containing 50 mL of test solution
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
ISO-medium.

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: A daily photoperiod of 16 hours


EFFECT PARAMETERS MEASURED:
Immobility (including mortality) At 24 hours and at 48 hours

RANGE-FINDING STUDY
- Test concentrations: WAFs prepared at loading rates of 1.0, 10 and 100 mg solid matter/L
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: No immobility was observed
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: 48h-EC50 was 0.59 mg/L
- Other: The sensitivity of this batch of D. magna was in agreement with the historical data collected at Charles River Den Bosch
Validity criteria fulfilled:
yes
Conclusions:
In conclusion, the 48h-EL50 for Daphnia magna exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 16 mg/L.
Executive summary:

The acute toxicity to Daphnia of the test item was assessed following OECD 202 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 48h-LL50 for Daphnia magna exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 16 mg/L.

Description of key information

The acute toxicity to Daphnia of the test item was assessed following OECD 202 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 48h-LL50 for Daphnia magna exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 16 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information