1-Octanol, reaction products with phosphorus oxide (P2O5), potassium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The experimental start date was 03 Aug 2018, and the experimental completion date was 15 Sep 2018.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

yes

Specific details on test material used for the study:

Purity: UVCB; solid matter 41.4%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: from the WAF and the control
- Sampling frequency: at t=0 h and t=96 h
- Sample storage conditions before analysis: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fraction (WAF) loading rate of 100 mg solid matter/L

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
- Length at study initiation: Range-finding test: 3.4 ± 0.3 cm, Limit test: 0.41 ± 0.11 cm
- Weight at study initiation: Range-finding test: 3.5 ± 0.2 g, Limit test: 0.52 ± 0.07 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water

ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions: same as test
- Type of food during acclimation: Daily with pelleted fish food

FEEDING DURING TEST
No feeding from 24 hours prior to the test and during the total test period

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg CaCO3 per litre

Test temperature:

20-24°C

pH:

pH 6.0-8.5

Dissolved oxygen:

> 60% of air saturation

Nominal and measured concentrations:

Loading: 100 mg solid matter/L
Measured: 22 mg solid matter/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 litres, all-glass, containing 9 litres of test solution
- Type: open
- Aeration: None
- No. of organisms per vessel: 7 fish per 9 litres
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.40 g fish/Litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED:
Mortality and other effects: At 2½, 24, 48, 72 and 96 hours following the start of exposure. In addition, every afternoon from day 0 to observe for any dead or severely distressed fish

TEST CONCENTRATIONS
Limit test: 100 mg solid matter/L

Reference substance (positive control):

yes

Remarks:

pentachlorophenol

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: no mortality observed
- Any observations that might cause a difference between measured and nominal values: Starting at 48h, the test solution was observed to be slightly hazy.

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- LC50: 96H LC50 0.15 mg/L
- Other: falls within the range of sensitivities generally observed during the
past years

Validity criteria fulfilled:

yes

Conclusions:

In conclusion, the 96h-LL50 for carp exposed to 1-Octanol, reaction products with phosphorus oxide (P2O5), potassium salts was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.

Executive summary:

The acute toxicity to fish of the test item was assessed following OECD 203 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 96h-LL50 for carp exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.

Description of key information

The acute toxicity to fish of the test item was assessed following OECD 203 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 96h-LL50 for carp exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L