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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
The experimental start date was 03 Aug 2018, and the experimental completion date was 15 Sep 2018.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
GLP compliance:
yes
Specific details on test material used for the study:
Purity: UVCB; solid matter 41.4%
Analytical monitoring:
yes
Details on sampling:
- Concentrations: from the WAF and the control
- Sampling frequency: at t=0 h and t=96 h
- Sample storage conditions before analysis: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fraction (WAF) loading rate of 100 mg solid matter/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
- Length at study initiation: Range-finding test: 3.4 ± 0.3 cm, Limit test: 0.41 ± 0.11 cm
- Weight at study initiation: Range-finding test: 3.5 ± 0.2 g, Limit test: 0.52 ± 0.07 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water

ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions: same as test
- Type of food during acclimation: Daily with pelleted fish food

FEEDING DURING TEST
No feeding from 24 hours prior to the test and during the total test period
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
180 mg CaCO3 per litre
Test temperature:
20-24°C
pH:
pH 6.0-8.5
Dissolved oxygen:
> 60% of air saturation
Nominal and measured concentrations:
Loading: 100 mg solid matter/L
Measured: 22 mg solid matter/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 litres, all-glass, containing 9 litres of test solution
- Type: open
- Aeration: None
- No. of organisms per vessel: 7 fish per 9 litres
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.40 g fish/Litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED:
Mortality and other effects: At 2½, 24, 48, 72 and 96 hours following the start of exposure. In addition, every afternoon from day 0 to observe for any dead or severely distressed fish

TEST CONCENTRATIONS
Limit test: 100 mg solid matter/L
Reference substance (positive control):
yes
Remarks:
pentachlorophenol
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Mortality of control: no mortality observed
- Any observations that might cause a difference between measured and nominal values: Starting at 48h, the test solution was observed to be slightly hazy.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- LC50: 96H LC50 0.15 mg/L
- Other: falls within the range of sensitivities generally observed during the
past years
Validity criteria fulfilled:
yes
Conclusions:
In conclusion, the 96h-LL50 for carp exposed to 1-Octanol, reaction products with phosphorus oxide (P2O5), potassium salts was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.
Executive summary:

The acute toxicity to fish of the test item was assessed following OECD 203 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 96h-LL50 for carp exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.

Description of key information

The acute toxicity to fish of the test item was assessed following OECD 203 under static conditions. Following a range-finding study, a limit test was performed with a WAF of the test item at 100 mg solid matter/L. No mortality or clinical effects were observed in both the control and the WAF during the test period. The 96h-LL50 for carp exposed to the test item was >100 mg solid matter/L. The analytically confirmed concentration of dioctyl phosphate in the test solution was 22 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

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