3-[(4-aminobenzoyl)amino]propanoic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04/07/2018 - 09/07/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

Batch: 12/18
Expiry date: 03:2019

Analytical monitoring:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Breeder in Eurofins Biollab test facility
- Age of parental stock: 6- 24 hours
- Feeding during test: None


ACCLIMATION
- Acclimation conditions: Temperature 20°C ± 2°C, photoperiod: 16 hours light/8 hours dark. water hardness: 140 - 250 mg CaCO3/l. Water change: 3 times per week.
- Type and amount of food: Green alga Pseudokirchneriella subcapitata and Saccharomices cerevisiae

Test type:

static

Water media type:

other: reconstituted water

Limit test:

yes

Total exposure duration:

48 h

Hardness:

between 140 and 250 mg CaCO3/L

Test temperature:

20°C ± 2°C
Results are shown in table 4 below

pH:

The results are shown in table 3 below

Dissolved oxygen:

> 3 mg/l
The full resutls are shown in table 2 below

Nominal and measured concentrations:

100 mg/l (nominal concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker (500 ml)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A dilution water, reconstitued water has been used by dissolving teh following salts in 1 L of deionsed water:
-Bihydrated calcium chloride: 11.7 g/L
-Eptahydrated magnesium sulphate: 4.93 g/L
-Acid sodium bicarbonate: 2.59 g/L
-Potassium chloride: 0.23 g/L

OTHER TEST CONDITIONS
- Photoperiod: None

EFFECT PARAMETERS MEASURED : Immobilisation


RANGE-FINDING STUDY
- Test concentrations: Ten fold dilutions from 100 mg/l to 0.01 mg/l
- Results used to determine the conditions for the definitive study: Yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Table 1: Number of immobilised Daphnia at 24 and 48 hours in treated (test item) and control groups in the definitive control:

Control	24 hours	48 hours
Control replication 1	0/5	0/5
Control replication 2	0/5	0/5
Control replication 3	0/5	0/5
Control replication 4	0/5	0/5
Concentration 1:100 mg/l
	24 hours	48 hours
Treated replication 1	0/5	0/5
Treated replication 2	0/5	0/5
Treated replication 3	0/5	0/5
Treated replication 4	0/5	0/5

Table 2: Concentration of dissolved oxygen (mg/l) in treated and control at the beginning and at the end of the test (48 hours) in the definitive test

Control	Beginning of test	End of test
Control replication 1	5.01	5.12
Control replication 2	5.12	5.17
Control replication 3	5.06	5.11
Control replication 4	5.14	5.20
Concentration 1:100.00 mg/l
Treated	Beginning of test	End of test
Treated replication 1	5.10	5.18
Treated replication 2	5.16	5.25
Treated replication 3	5.21	5.28
Treated replication 4	5.15	5.21

Table 3: Determination of pH at the beginning and at the end of the test (48 hours) in treated and control in the definitive test

Control	Beginning of test	End of test
Control replication 1	7.72	7.79
Control replication 2	7.72	7.83
Control replication 3	7.73	7.81
Control replication 4	7.70	7.77
Concentration 1:100.00 mg/l
Treated	Beginning of test	End of test
Treated replication 1	6.33	6.52
Treated replication 2	6.35	6.54
Treated replication 3	6.35	6.51
Treated replication 4	6.34	6.56

Table 4: Determination of temperature (°C) in eth environment where the assay is performed at the beginning and at the end of the test ( 48 hours) in the definitive test

Time	Temperature
Beginning of test	20.2
End of test	20.1

Validity criteria fulfilled:

yes

Conclusions:

The obtained results, in compliance with assay validity criteria showed that Daphnia magna EC50 after 48 hours of the nominal concentraiton of the test item is > 100 mg/L

Description of key information

A GLP study was performed in accordance with OECD Guideline 202 to assess the toxicity of the test material, 4 -aminobenzoyl-b-alanine, to Daphnia magna. According to the results of the test, the EC50 (48 h) was > 100 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L