3-methyl-1,3-butanediol-1-ethylate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011-05-09 to 2011-06-06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
Lot number: KM1035
Impurity: identity unknown (0.6613%)
purity: 99.3387%

Oxygen conditions:

aerobic/anaerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- The basal medium was prepared by adding 6 mL each of the solutions A, B, C, and D to 1976 mL of purified water (Pharmacopeia of Japan/JP) and adjusted to pH 7.0.
- Method of cultivation: in thermostatic chamber (temperature: 25 ± 1°C)
- Concentration of sludge: 30 mg/L, no pH adjustment
- MLSS measurement: MLSS: 3425 mg/L
- Water filtered: yes

Activated sludge solution was prepared by diluting with the basal medium to 900 mg/L based on the concentration of suspended material in the activated sludge culture solution.

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 25 +/- 1 °C
- pH: 7.0
- pH adjusted: yes - A given amount of the basal medium or purified water (JP) was added to adjust to pH 7.0

TEST SYSTEM
- Culturing apparatus: Coulometer OM-3100A
- Number of culture flasks/concentration: 3
- Test performed in closed vessels

SAMPLING
- Sampling frequency: day 7, 14, 21 and 28
- Sampling method: After the 28-day culture period, except the vessel for degradation of aniline, 10 mL of the test solution is pipetted with a transfer pipette and centrifuged (4000 g, 15 min). Then, 7 mL of the supernatant was used as a sample for measurement of the concentration of DOC.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Other: Vessel for stability in water

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

>= 86

Sampling time:

28 d

Details on results:

Test substance undergoes 86% degradation by BOD in 28 days.

Parameter:

BOD5

Value:

56.1 mg O2/g test mat.

Parameter:

COD

Value:

17.2 mg O2/g test mat.

Remarks on result:

other:

Remarks:

water + test substance system

Results with reference substance:

BOD (day 28): 78.5 mg

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance, 3-Methyl-1,3-butanediol acetate, underwent biodegradation and then was mineralized. Therefore, the test substance is determined to be ready biodegradable.

Description of key information

Biodegradability of 3-Methyl-1,3-butanediol acetate was tested in triplicates for 28 days. The test substance undergoes 86 % degradation in 28 days, determined by BOD. Based on percentage degradation, 3-Methyl-1,3-butanediol acetate was estimated to be readily biodegradable in water.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

[Type of water: freshwater]