Reaction mass of carbon tetrachloride and chloroform and 1,2-dichloroethane

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Even if the data are only available from secondary source (http://www.safe.nite.go.jp/english/kizon/KIZON_start_hazkizon.html and IUCLID 4 file), the data set has been evaluated by U.S. Environmental Protection Agency and the CTC BCF value of 7.4 data from this source were integrated in the EPI suite 4.0 training set for the BCFBAF™ software.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Principles of method if other than guideline:

Test Equipment: Improved type for a volatile substance

GLP compliance:

not specified

Radiolabelling:

no

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Test temperature:

22 °C

Dissolved oxygen:

6-8 mg/l

Reference substance (positive control):

not required

Lipid content:

3.4 %

Type:

BCF

Value:

>= 3.2 - <= 7.4

Basis:

total lipid content

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:10 ppb (µg/L)

Type:

BCF

Value:

>= 3.8 - <= 11

Basis:

total lipid content

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:1 ppb (µg/L)

Executive summary:

Bioconcentration factors (BCF) for CTC have been measured for CTC following the OECD 305C. For Cyprinus carpio BCF 3.2 - 7.4 were determined at with a test concentration of 1 ppb (µg/l) and BCF 3.8 - 11 with a test concentration of 10 ppb (µg/l).

Description of key information

Based on data available, CTC is considered as not bioaccumulable and does not fulfil B criterion of PBT assessment.

BCF value: 11

Key value for chemical safety assessment

BCF (aquatic species):

11 dimensionless

Additional information

No experimental study was conducted on the multiconstituent substance (Flux1). Instead, a constituent-based, Weight-of-Evidence approach, was performed.

The three major constituents were targeted, representing ca 95% of a typical composition (carbon tetrachloride (CAS n° 56-23-5),1,2-Dichloroethane (CAS n°107 -06 -2) and chloroform (CAS n° 67-66 -3)). Numerous published data were available, and a single consensus value was selected for each, from a reliable source.

No average key value was derived for the multiconstituent substance: as the Risk Assessment is conducted per constituent, individual values are the key data.

Bioconcentration factors (BCF) for carbon tetrachloride (CTC) have been measured following the OECD 305C (MITI Japan, 1992). BCF values of 3.2 - 7.4 and 3.8 - 11 were determined for Cyprinus carpio with a test concentration of 1 ppb and 10 ppb (µg/L) of CTC, respectively. These results showed that CTC is not bioaccumulable.

CTC is considered as not bioaccumulable and does not fulfil B criterion of PBT assessment.