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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From October 29, 2020 to November 02, 2020
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
18 June 2019
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L
- Sampling method: samples were taken from test group and control group of fresh media at 0 hour fresh solutions and 96 hours old solutions.
- Sample storage conditions before analysis:
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.5007 g test item was weighed and dissolved in 5 L of test water by magnetic stirring for about 2 hours. After the test item was dissolved completely, the solution was used for exposure. 5 L test water was used for control group.
Test organisms (species):
other: Rare Minnow (Gobiocypris rarus)
Details on test organisms:
TEST ORGANISM
- Common name: Rare Minnow
- Strain: (Gobiocypris rarus)
- Source: CTI Chemical Safety Evaluation Research Center (SUZHOU) CO., LTD.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Twice on weekday and one time on weekend until 24 hours before the test was started
- Health during acclimation (any mortality observed): From hatching, there were not any signs of disease or significant numbers of mortality.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
40 to 250 mg/L (as CaCO3)
Test temperature:
23±2oC
pH:
6.0 to 8.5 pH units
Dissolved oxygen:
Not less than 60% of air saturation value (ASV) during the test period
Nominal and measured concentrations:
Range-finding test: 10 and 100 mg/L;
Definitive test: 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Rectangle glass tanks (length: 30 cm, width: 20 cm, height: 30 cm)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration: Before usage, the water will be aerated to make dissolved oxygen concentration meet requirement of test.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: loading <0.8 g wet weight fish/L water

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours darkness and an intensity of 540-1000 lux
- Light intensity: 590 ~ 680 lux

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no visible toxic signs.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
During the 96-hour exposure period, no mortality and visible toxicity were observed in the control group and test group of 100 mg/L.
During the test period, chemical analysis on test item concentration in test media by GC-MS/MS showed that for the test media of 100 mg/L, the measured concentration of fresh media was 95.2 mg/L. The measured concentration of old media was 93.9 mg/L. The mean concentration was 94.5 mg/L, which was 94.5% of nominal concentration.
Validity criteria fulfilled:
yes
Conclusions:
The 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.
Executive summary:

A fish acute toxicity test of test item was conducted according to OECD 203 under GLP, with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period.


Based on the results of the preliminary test, the definitive test was carried out at 1 limit nominal concentration of 100 mg/L.


According to the results and based on the nominal concentration, the 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.

Description of key information

The 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 100 mg/L

Additional information

A fish acute toxicity test of test item was conducted according to OECD 203 under GLP, with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period.


Based on the results of the preliminary test, the definitive test was carried out at 1 limit nominal concentration of 100 mg/L.


According to the results and based on the nominal concentration, the 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.