Potassium tris(pentafluoroethyl)trifluorophosphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24/09/2007-29/11/2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test. and Reproduction Test".

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Chemical Name: Potassium tris(pentafluoroethyl)trifluorophosphate
Supplier: Merck KGaA
Batch: S4693525
Specification: ionic liquid
Appearance: off white, crystalline powder with grey tinge
Expiration date: 30 Nov 2007
Strorage: at room temperature, in tightly closed brown-glass container protected from light and moisture
Purity (NMR): >98 % (w/w)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
all test concentrations and the control

- Sampling method:
Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the first treatment period (Day 0) and after a period of 48 h.

- Dilution: 1/1 (v/v) with pure water

Test solutions

Vehicle:

yes

Details on test solutions:

Macro nutrients (mg/L)
CaCI2 •2H20 293.80
MgSO4•7H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3•9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18

Trace elements mg/L
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003

Macro nutrients
Na2EDTA • 2H20 2.50

Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75

- pH: 8 ± 0.3
- hardness 14 ° dH (250 mg/L CaCO3)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain/clone: Daphnia Magna
- Source: Merck KGaA
- Age of parental stock: younger than 24 hours
- Feeding during test
- Food type: green algea (Scenedesmus subspicatus)
- Amount: was based on the concentration of total organic carbon (TOC) in the algal food suspension.
- Frequency: once per week


ACCLIMATION
- Acclimation period:
- Acclimation conditions: same as test (controlled environment room, pH 7.9-8.0, 370-400 lux, 16h light / 8 h dark)
- Type and amount of food: green algea (Scenedesmus subspicatus)
- Feeding frequency: once per week
- Health during acclimation : no mortality observed


At the start of the experimental phase 5 Daphnia magna were placed in each test vessel containing 10 ml reconstituted water with or without test material. The test vessels were left open.

Study design

Test type:

other: open-static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

14o dH (about 250 mg/L CaCO3)

Test temperature:

19 – 20°C

pH:

7.9 +/- 0.3

Dissolved oxygen:

at least 90 %

Nominal and measured concentrations:

Nominal / measured (in mg/L)
0.05 / LOQ, 0.24 / > LOQ, 0.53 / > LOQ, 1.17 /> LOQ, 2.58 / 2.57, 5.67 / 5.34, 12.47 / 12.54

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open static
- Material: test item in reconstituted water
- Aeration: 24 hours after preparation
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: --


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted according to ELENDT (1990)



Macro nutrients mg/L
CaCl2 x 2H2O 293.80
MgSO4 x 7H2 123.30
NaHCO3 64.80
KCl 5.80
Na2SiO3 9H2O 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18


Trace elements mg/L
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients
Na2EDTA  2H2O 2.50

Vitamins µg/L
Thiamine 75.00
B12 1.00
Biotin 0.75


- Total organic carbon: --
- Particulate matter: --
- Metals: --
- Pesticides: --
- Chlorine: --
- Alkalinity: --
- Ca/mg ratio: --
- Conductivity: --
- Culture medium different from test medium: no
- Intervals of water quality measurement: 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: 7.9
- Photoperiod: 16h light / 8h dark
- Light intensity: --


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

References:

ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

other: mortality

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 0.43-0.81

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: mortality

Effect conc.:

0.21

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 0.17-0.27

Details on results:

In this open static test system, Daphnia magna exposed to nominal concentrations of 0.11 up to 12.47 mg/L led to immobilized daphnia. The Concentration of 0.05 mg/L revealed no biological effects.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Potassium tris(pentafluoroethyl)trifluorophosphate, solved in reconstituted water, was tested in an open static test system. Under the given conditions of this study the 48 h EC50 value was 0.21 mg/L.

Executive summary:

The objective of this study was to determine the acute toxicity of the test material using Daphnia magna.

For this purpose, maximally 24 hours old Daphnia magna were exposed to aqueous test material concentrations under defined conditions.

The study comprised of four test vessels per concentration containing five Daphnia magna, i.e. 20 Daphnia per concentration (test medium group)

and control group. The Daphnia were observed for immobilization up to 48 hours.

Daphnia magna were exposed to the reconstituted water or to test material concentrations of nominal 0.05, 0.11, 0.24, 0.53, 1.17, 2.58, 5.67 and 12.47 mg/L in an open static system.

The nominal concentrations from 0.11 up to 12.47 mg/L led to immobilized daphnia. The concentration of 0.05 mg/L revealed no biological effects.

The EC50 values with the 95 % confidence intervals based on the nominal concentrations are given below.

24 h ECS0 0.60 (0.43 - 0.81) mg/L

48 h ECS0 0.21 (0.17 - 0.27) mg/L

The test item solved in reconstituted water, was tested in an open static assay. Under the given conditions of this study the 48 h ECS0 value was 0.21 mg/L.