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EC number: 233-357-8
CAS number: 10127-27-2
after 240 Minutes Treatment Time
Proposed in vitro Irritancy Score
Aluminium Orange G
# Opacity value = Difference (t240
- t0) of Opacity
## Permeability at 490 nm (OD490)
in vitro study was performed to assess the corneal
irritation and damage potential of the test item by
means of the BCOP assay using fresh
a first opacity measurement of the fresh bovine corneae (t0),
the 20% (w/v)
suspension in saline (0.9% (w/v) NaCl in deionised water) of the test
item as well as the positive and the negative controls were each applied
to different corneae fixed in an incubation chamber in horizontal
position and incubated for 240 minutes at 32 ± 1 °C.
chamber contained incubation medium. After the incubation phase the test
item, the positive, and the negative controls were each rinsed from the
was measured again (t240).
the opacity measurements, permeability of the corneae was determined by
measuring spectrophotometrically the transfer of sodium fluorescein
after incubation in a horizontal position for 90 minutes at 32 ± 1 °C.
the negative control (physiological saline), neither an increase of
opacity nor permeability of the corneae was observed.
positive control (10% (w/v) benzalkonium chloride in saline) showed
clear opacity and distinctive permeability of the corneae corresponding
to a classification as serious eye damage (EU CLP/UN GHS Category 1).
to the negative control, the test item caused an increase of the corneal
opacity or permeability. The calculated mean in vitro irritancy
score was 58.55.
to OECD 437 the test item is classified as serious eye damaging (EU
CLP/UN GHS Category 1).
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