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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one
EC Number:
819-558-9
Cas Number:
2020359-04-8
Molecular formula:
C29H39NO2
IUPAC Name:
1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one
Test material form:
solid: particulate/powder
Details on test material:
a off-white powdery solid, without irritating odor

Study design

Test type:
not specified
Water media type:
other: tap water
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20.4 – 21.2 °C
pH:
7.0
Details on test conditions:
Duration 3 hours
Replicates 1 replicate/concentration (positive control, test item lower concentrations)
5 replicates for test item treatment 1000 mg/L
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water, see annex 2, page 22
Aeration purified air, using Pasteur pipettes
Feeding nutrient solution, 16 mL/vessel
Temperature 20.4 – 21.2 °C
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol (CAS-No. 591-35-5)

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
One experiment was performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Because no significant inhibition was observed, no additional experiment had to be per-formed.
All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The positive control gave an EC50 of 7.4 mg/L (95 % confidence interval not determinable) which lies within the recommended range of 2 – 25 mg/L. The coefficient of variation of oxygen uptake rate in control replicates was below 30% at the end of the test. The oxygen uptake rate of the blank controls was above 20 mg O2 per gram activated sludge in 1 hour.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes