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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report Date:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
a off-white powdery solid, without irritating odor

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
In the Daphnia sp. breeding room of Centralbio Co., Ltd.

Study design

Test type:
static
Water media type:
not specified
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1°C
pH:
6.00 ~ 9.00.
Dissolved oxygen:
not less than 3 mg/L
Nominal and measured concentrations:
1.0, 10.0 and 100.0 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7).

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.006 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.006 mg/L
Details on results:
During the exposure period, the percent immobilisation in the control group was less than 10 %. No abnormal signs or behaviors such as discoloration and trapping of Daphnia magna at the surface of water were evident in the control group. In addition, the dissolved oxygen concentrations in the control group and treatment group were 3 mg/L or more at the end of exposure. Therefore, the results were considered to be acceptable.
Based on the range finding test, limit test was performed in control, and 100.00 mg/L. As a result of the limit test, The geometric mean measured concentration of the test substance at the 100.00 mg/L was 0.00611 mg/L during test period.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
During an exposure period, there was not immobilisation and death at limit test. Therefore EC50 was reported >0.00611 mg/L and NOEC was reported ≥0.00611 mg/L. All results of test were determined based on geometric mean measured concentration.