methylethylketone peroxide trimer

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07-2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study is done under GLP and considered complete

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

All test solutions were prepared separately. Specific loading rates of the test substance were added to M7 medium and stirred for 48 hours. The resulting mixtures were stabilized for 24 hours in a separation funnel after which the Water Accommodated Fractions (WAFs) were
obtained using the drain cock.

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Details on test conditions:

The reproduction test was performed in closed vessels in a semi-static system. It included 10 vessels per test concentration and 20 vessels for an untreated control group. Each of the vessels contained one neonate «24h old) Daphnia magna in 50 ml test medium. The nominal
concentrations of Trigonox 301 tested were 1.0, 1.8, 3.2, 5.6 and 10 mg/L. The study duration was 21 days and the test solutions were renewed every 48 hours. The daphnids were fed on a daily basis with a Chtorella pyrenoidosa suspension.

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

3.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

> 2.7 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

Two out of the twenty parental daphnids in the control died during the test period. Mortality in the Trigonox 301 treatments of 0.93, 1.8 and 2.7 mg/l was comparable to or lower than the control and not statistically significant. 90-100% of the daphnids exposed to 4.9 and 8.3 mg/L, respectively, died during the test. Hence, the mortality of the parental daphnids was clearly treatment related and followed a very steep dose curve.

The average cumulative number of young per female in the control after 21 days was 120 ± 13. The reproduction curve recorded at 0.93, 1.8 and 2.7 mgtl closely followed or was even slightly above the curve of the control. The curves recorded at the higher concentrations clearly stayed behind from the beginning of reproduction. Since these higher concentrations of 4.9 and 8.3 mg/L showed a high mortality number of parental organisms no final judgement regarding reproduction was possible. There were no recordings of unhatched (aborted) eggs at any of the test concentrations throughout the 21-day test period. Immobile young were only recorded at 4.9 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

study is complete and valid

Description of key information

One OECD 211 GLP test is available with an overall NOEC of 2.7 mg/L (mean measured concentration)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.7 mg/L