1,3-Cyclopentanedione, 2-[(2E)-2-(3,4-dihydro-6-methoxy-1(2H)-naphthalenylidene)ethyl]-2-ethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Sept 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-Methoxy-18-methyl-8,14-seco-1,3,5(10),9(11)-estratetraene-14,17-dione

EC Number:

613-005-3

Cas Number:

62298-52-6

Molecular formula:

C20H24O3

IUPAC Name:

3-Methoxy-18-methyl-8,14-seco-1,3,5(10),9(11)-estratetraene-14,17-dione

Details on test material:

- Name of test material (as cited in study report): 17-Keto-Ethyl-Seco_Verbindung
- Analytical purity: 98.8%
- Lot/batch No.: 10037206

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

No immobilized Daphnia were observed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50/48 hours of ZK 46073 to Daphnia magna is higher than a saturated solution (7 mg/L) .

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of 17 -Keto-Ethyl-Seco-Verbindung (ZK 46073) to Daphnia magna in a limit-test. The study was conducted in agreement with thetest guideline of the OECD, no. 202, "Daphnia spec., acute immobilization test".

Twenty Daphnia were used for the test solution of ZK 46073 and twenty for the control group. The Daphnia were exposed to the test substance and the dilution water for aperiod of 48 hours under static conditions in a limit-test. Immobilization was recorded at 24 hours and 48 hours. In order to establish a saturated solution, a suspension of 100 mg/L was prepared and stirred tor 24 hours. Then it was filtered through a glassfibre filter. This saturated solution was used for the test. Samples for a TOC analysis were taken at 0 and 48 hours. The TOC corresponded to a concentration of 7 mg/L, calculated on the basis of the structural formula of ZK 46073.

No immobilized Daphnia were observed.