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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly added to the test medium

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge from laboratory waste water treatment plants treating municipal and synthetic sewage
- Initial biomass concentration: 1 g/l

Study design

Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2 °C
pH:
6.5 - 6-7 (Before correction)
7.1 - 7.5 (After correction)
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
20,
100,
250,
500,
1000,
control
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer vessels (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium (8 ml/vessel, 100-fold concentrated OECD medium)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenole

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
45 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion