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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium at 20 °C for ca. 20 h. Undissolved test substance was removed by centrifugation (20 min at 17700 g)
- Eluate: The nominal concentration of the eluate was 100 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h
- Feeding during test. none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.42 mmol/l
Test temperature:
20.2 - 20.6 °C
pH:
7.7 - 8.0
Dissolved oxygen:
7.8 - 8.4 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
1.56,
3.13,
6.25,
12.5,
25,
50,
100,
control

Analytical test concentrations (mg/l) for nominal 3.13, 25 and 100 mg/l:
1.1,
8.8,
34.7
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom test tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ISO 10706
- Ca/mg ratio: 4:1
- Conductivity: 613 µS/cm
- Intervals of water quality measurement: after 0 and 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 day-night regime
- Light intensity: Artificial light, type warm white ( e.g. OSRAM L 58 W31), ca. 2 -7 µE(m²-s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
2.22 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
35.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Recovery rate of the test substance < 80 %
Reported statistics and error estimates:
Probit analysis

Applicant's summary and conclusion