Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from laboratory waste watper treatment plant treating municipal and synthetic sewage.
- Concentration of sludge: 6 mg/l
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
36.3 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: dionised water (1000 ml), inorganic medium (19.5 ml), inoculum (7.5 ml), test substance (54.4 ml) filled up to a total volume of 1500 ml with dionised water.
- Test temperature: 22 +/- 2 °C
- pH: 7.4 (before adjustment 7.6 - 7.7)
- pH adjusted: yes


TEST SYSTEM
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: daily


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
40 - 50
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
80 - 90 % degradation after 14 d

Applicant's summary and conclusion