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EC number: 922-520-5 | CAS number: -
Table 1: Final Test: Test Samples Analysis Results
Time of sampling[hours]
Loading rate (a)[mg/L]
< LOD (b)
(a) A water accommodated fraction (WAF)prepared at the loading rate.
(b) The limit of detection (LOD) of the analytical method was determined to be 0.00078 mg/L taking a dilution factor of two into account.
(c) Concentrations were calculated by extrapolation of the calibration curve. Hence these samples were re-diluted and analyzed the next day.
n.a. Not applicable.
Table 2: Number of Introduced Daphnids and Incidence of Immobility in the Final Test
Average exposure conc. (mg/L)
#Microscopic observation revealed no test item attached to the daphnids.
In a semi-static 48 h acute toxicity test conducted according to OECD guideline 202, Daphnia magna were exposed to Water Accommodated Fractions (WAFs) of the test substance individually prepared at loading rates of 0.47, 1.0, 2.2, 4.6 an 10 mg/L and an untreated control (all with 4 replicates per concentration and 5 daphnids per replicate). Concentrations measured in the freshly prepared solutions were below LOD in the two lowest treatments, while a dose related increase was observed at the higher test concentrations. Measured concentrations decreased by more than 27% during both refreshments. Therefore, averaged geometric mean measured exposure concentrations were used to determine the effect parameters and were calculated to be 0.00039, 0.00039, 0.55, 2.0 and 4.2 mg/L.
No immobility was observed in the control and the two lowest test concentrations throughout the exposure period. At the end of the test, 30% immobility was observed at 0.55 mg/L, while total immobility was observed at the two highest test concentrations.
The 48h-EC50 for immobility was 0.66 mg/L based on average exposure concentrations (95% confidence interval between 0.44 and 0.99 mg/L).
The study met all validity criteria and is considered to be reliable without restrictions.
The 48h-EC50 of the substance to Dapnhia magna was determined to be 0.66 mg/L, based on average exposure concentrations (95% confidence interval 0.44 - 0.99 mg/L).
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