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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Publication; test procedure comparable to guideline

Data source

Reference
Reference Type:
publication
Title:
Toxicity of 58 substituted anilines and phenols to algae Pseudokirchneriella subcapitata and bacteria Vibrio fischeri: Comparison with published data and QSARs
Author:
Aruoja, V.
Year:
2011
Bibliographic source:
Chemosphere 84 (2011) 1310–1320

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-xylenol
EC Number:
202-461-5
EC Name:
2,5-xylenol
Cas Number:
95-87-4
Molecular formula:
C8H10O
IUPAC Name:
2,5-dimethylphenol
Test material form:
not specified
Details on test material:
- Name of test material: 2,5-dimethylphenol (2,5-DMP)
- Molecular weight:122.2
- Analytical purity: > 99 %
- Hydrophobicity (log Kow): 2.33

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
All test sulutions were prepared in algal test medium.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: P. subcapitata
- Source: commercial test system Algal Toxkit F (MicroBioTests Inc., Nazareth, Belgium)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
24 ± 1 °C
pH:
8.0 ± 0.5
Details on test conditions:
TEST SYSTEM
- Test vessel: 20-mL glass scintillation vials
- Initial cells density: 10000 cells /mL after inoculation
- No. of vessels per concentration: 2
- No. of vessels per control: 8


GROWTH MEDIUM
- Standard medium used: yes



OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: yes
- Light intensity and quality: vials were illuminated from below with Philips TL-D 38W aquarelle fluorescent tubes


EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: optical density at 682 nm directly from the incubation vials (spectrophotometer)


TEST CONCENTRATIONS
- Test concentrations: 6 or 7 concentrations, depending on previously available toxicity data from literature or preliminary experiments
Reference substance (positive control):
yes
Remarks:
Aniline

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
32.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The determined EC50 values of the test item in P. subcapitata 72-h growth inhibition was 32.5 mg/L.
Results with reference substance (positive control):
The determined EC50 values of P. subcapitata 72-h growth inhibition was 54.2 mg/L.

Applicant's summary and conclusion