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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
441-070-6
EC Name:
-
Cas Number:
61826-76-4
Molecular formula:
C14H11NO2
IUPAC Name:
(2S)-2-hydroxy-2-(3-phenoxyphenyl)acetonitrile

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
water
Details on test solutions:
Immediately before the beginning of the test a 20 mg/L solution was prepared by dissolving 5.2 mg test substance in 250 mL reconstituted water. The appropriate concentrations were then obtained by diluting with reconstituted water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Neonates of Daphnia magna, less than 24 hours old, were obtained by hatching ephippia, supllied in the microbiotest Daphtoxkit F.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg CaCO3/L
Test temperature:
20 °C
Nominal and measured concentrations:
Nominal concentrations: 0, 0.10, 0.18, 0.32, 0.56 and 1.00 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Comments:
Due to the rapid hydrolysis the determination of the test substance concentration was not possible. At the beginning of the test, the test substance concentrations were already below the detection limit of 0.2 mg/L. The concentrations of the hydrolysis product were therefore also determined and were stable between the begin and the end of the test.

Results:

- Immobilisation of control group daphnids was 0% at the end of the tests (guideline maximum of 10%)

- There were no control daphnids trapped at the water surface

- Temperature was stable during the test period

- Dissolved oxygen concentrations in the test vessels were not lower than 8.5 mg/L (EC guideline: higher than 3.5 mg/L)

- The concentrations of the hydrolysis product were stable between the begin and the end of the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
For the static acute immobilization test with Daphnia magna conducted according to EU method C.2 the following effect concentrations were determined after treatment with the test item:
- 48 h EC50: 0.9 mg/L (95% confidence limit (upper 1.2, lower 0.8)
- 48h EC0: 0.6 mg/L.
Executive summary:

For the static acute immobilization test with Daphnia magna conducted according to EU method C.2 the following effect concentrations were determined after treatment with the test item:

- 48 h EC50: 0.9 mg/L (95% confidence limit (upper 1.2, lower 0.8)

- 48h EC0: 0.6 mg/L.