Registration Dossier
Registration Dossier
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EC number: 205-741-5 | CAS number: 149-45-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7 Dec 2016 - 12 Jan 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
Test material
- Reference substance name:
- Disodium 4,5-dihydroxybenzene-1,3-disulphonate
- EC Number:
- 205-741-5
- EC Name:
- Disodium 4,5-dihydroxybenzene-1,3-disulphonate
- Cas Number:
- 149-45-1
- Molecular formula:
- C6H6O8S2.2Na
- IUPAC Name:
- disodium 4,5-dihydroxybenzene-1,3-disulphonate
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf. Date of collection: 2016-12-09
- Pretreatment: The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use.
- Concentration of sludge: The dry matter was determined as 4160 mg suspended solids/L. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 90 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: OECD guideline test medium
- Test temperature: 18.7 – 21.7 °C
- pH: 6.3 - 7.3
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 4160 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2000 mL-SCHOTT-flasks were used as test vessels, 100 mL scrubber flasks as absorbent vessels.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with purified (by activated charcoal), CO2-scrubbed, moistened air.
- Measuring equipment: Carbon analyser TOC multi N/C 2100S, Analytik Jena
- Test performed in open system: no
SAMPLING
- Sampling frequency: day 0, 2, 4, 7, 9, 11, 15, 17, 23 and 29
- Sampling method: 10 samples of 1 mL were taken from each flask.
- Sterility check if applicable: no
- Sample storage before analysis: not stated
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic control: yes
- Toxicity control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9.2
- Sampling time:
- 29 d
- Remarks on result:
- other: mean of two flasks
- Details on results:
- The test item is considered as not readily biodegradable. The degree of biodegradation reached 9% after 28 days and the 10-day-window was not met.
Abiotic degradation reached 15.7 %.
BOD5 / COD results
- Results with reference substance:
- 89.7% after 29 days
Any other information on results incl. tables
Biodegradation results [%]:
| Day | Positive Control 1 | Positive Control 2 | Positive Control Mean | Test 1 | Test 2 | Test Mean | Abiotic Control | Toxicity Control |
| 2 | -0.4 | 1.3 | 0.5 | -0.3 | 2.5 | 1.1 | 6.4 | 0.5 |
| 4 | 21.5 | 24.2 | 22.9 | 2.3 | 2 | 2.1 | 7.2 | 2.7 |
| 7 | 60.9 | 66 | 63.4 | 0.2 | 0.3 | 0.3 | 7.9 | 16.3 |
| 9 | 72.3 | 81.2 | 76.8 | 2.5 | 5 | 3.7 | 8.6 | 23.9 |
| 11 | 77.3 | 84.9 | 81.1 | 1.1 | 5.1 | 3.1 | 8.4 | 26.6 |
| 15 | 80.9 | 87.4 | 84.2 | 0.9 | 5.7 | 3.3 | 8.1 | 30 |
| 17 | 85.8 | 95 | 90.4 | 2.4 | 8.9 | 5.6 | 2.9 | 32.6 |
| 23 | 86.2 | 90.4 | 88.3 | 1.8 | 10.1 | 6 | 9 | 33.7 |
| 29 | 87.2 | 92.2 | 89.7 | 5.9 | 12.5 | 9.2 | 15.7 | 40.4 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
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