Fatty acids, soya, iso-Pr esters

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: National Guideline with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Guideline issued by Ministry of Agriculture, Fisheries and Food, United Kingdom, AEP2, 1984

Principles of method if other than guideline:

96-h exposure of marine species Crangon crangon to 5 concentrations of the test substance under semi-static conditions. Mortality is recorded.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl oleate

EC Number:

203-935-4

EC Name:

Isopropyl oleate

Cas Number:

112-11-8

Molecular formula:

C21H40O2

IUPAC Name:

isopropyl octadec-9-enoate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since the test material was immiscible with water, constant stirring of test solution was applied to ensure a reasonable amount of test substance remained in suspension. The tests animals where shielded from the stirrer.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes, a floating layer above the test solution

Test organisms

Test organisms (species):

Crangon crangon

Details on test organisms:

TEST ORGANISM
- Common name: Brown shrimp
- Source: wild cought by Mr. R.J. Pipe in Burnham-on-Crouch, Essex; specimen collected in lower estuary, River Crouch, on 27th Feb 1991
- Weight at study initiation: mean weight 1.072 g ± 0.221 g
- Length at study initiation: mean length 50.9 mm ± 3.285 mm
- Feeding during test: no

ACCLIMATION/HOLDING
- Acclimation conditions: at least two days holding prior the test in artificial seawater (Marinemix), formulated by Wiegandt GmbH in the same temperature range as in main test
- feeding during acclimation: minced ox heart (approx. 1% of body weight per day)
- no further data on conditions and observations during acclimation are provided

Study design

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

14.48 °C (mean)

pH:

7.8 - 8.4

Dissolved oxygen:

83 - 89% (air saturation value)

Nominal and measured concentrations:

nominal: 850, 1530, 2640, 4760 and 8500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L glass aquaria
- Aeration: constant; air continuously diffused through the aquaria
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Artificial seawater (Marinemix), formulated by Wiegandt GmbH

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: mortality after 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Range finding study: was conducted, no details given

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 8 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- No mortality observed at all concentrations (0 - 8500 mg/L) during the test period of 96 hours.
- Part of the test sample was observed to remain on the liquid surface, even with continuous stirring. The agitation was sufficient to maintain a distribution within the body of the liquid, but at higher concentrations the thickness of the floating layer increased. Thus concentrations above 1% v/v were not evaluated.