Reaction products of 3-aminomethyl-3,5,5-trimethylcyclohexylamine with 2,2'-[(1-methylethylidene)bis(4,1-phenyleneoxymethylene)]bisoxirane

Endpoint summary

Additional information

Hydrolysis

The hydrolysis was examined at the two different pH values 4.0 and 9.0 in the dark at different temperatures of 50 °C (± 0.5), 55 °C (± 0.5) and 67 °C (± 0.5). The hydrolysis reaction was carried out using a dark thermostat to avoid photolytic effects. Nitrogen was bubbled into the water for five minutes before the preparation of the solutions in order to exclude oxygen. The calculated half-lives of the hydrolysis are 236 days (5659 hours) at temperature 25 °C and pH 4.0, 13 days (305 hours) at temperature 50 °C and pH 4.0, 54 days (1299 hours) at temperature 25 °C and pH 9.0 and 5 days (122 hours) at temperature 50 °C and pH 9.0.

Ready biodegradability

The ready biodegradability of the test item was studied in a closed bottle test. The test item was exposed to activated sludge for 28 days. Under test conditions the percentage biodegradation of the test item reached a mean of 2.3 % after 28 days based on the ThODNO3. Therefore, the test item can be considered to be not readily biodegradable. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThODNO3.

Adsorption/desorption

The adsorption/desorption characteristics of the test item were studied using high perfomance liquid chromatography. While passing through a column along with the mobile phase the test item interacts with the stationary phase. As a result of portioning between mobile and stationary phases the test item is retarded. The dual composition of the stationary phase having polar and non-polar sites allows for interaction of polar and non-polar groups of a molecule in a similar way as in the case for organic matter in soil or matrices. This enables the relationship between the retention time on the column and the adsorption coefficient on organic matter to be established. For calibration purposes the following reference substances were used:

Log Koc mean values of reference substances used from the batch equilibrium method as follows:

Acetanilide = 1.25

Methyl benzoate = 1.80

Isoproturon = 1.86

Linuron = 2.59

Naphthalene = 2.75

Pyrazophos = 3.65

Phenantrene = 4.09

DDT = 5.63

The reference substances were dissolved in methanol. The concentration of the stock solutions was about 1 mg/mL. These stock solutions were diluted with the eluent resulting in concentrations of the test solutions of approximately 50 µg/mL. The test item was dissolved in methanol. The concentration of this solution was about 1 mg/mL. It was diluted with eluent to 500 µg/mL. Three series were measured which contained the above reference solutions and the test item solution. The series of the solution were measured with two parallel injections, each. The retention times were determined. The three calibration series and three test item series were measured alternately. For the determination of t0 (t0 = retention time of the unretarded component) Sodium Nitrate solution was measured three times. Chromatograms were evaluated using LaChrom chromatogram processor software. Calculations were carried out using EXCEL for Windows. The capacity factors, k’ were calculated from the retention times (tR) of the selected reference substances and the dead time (t0). The log k’ data of the reference substances were plotted against their log Koc values. The regression coefficients (m, b) were determined by linear regression calculation using the least squares method. Log Koc value of the test item was calculated using these regression coefficients and the capacity factor of the test item: log k' = m * log Koc + b. The log Koc was found to be 4.82. The quality criteria were met, as values of log Koc derived from individual measurements fell within a range of ± 0.25 log units and the correlation coefficient R was >0.95.