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Toxicity to terrestrial plants

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Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable, well documented study
Qualifier:
according to guideline
Guideline:
OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on sampling:
no data available
Vehicle:
not specified
Details on preparation and application of test substrate:
A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:

pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 %
Species:
other: Nicotiana tabacum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tobacco plants (N. tabacum SR-1) were used for the experiment.
Test type:
vegetative vigour test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d
Remarks:
approximately 3 weeks
Post exposure observation period:
no data available
Test temperature:
25/20 °C light/dark temperature regime
pH:
6.40 - 6.83
Moisture:
60% relative humidity
Details on test conditions:
16 h light period (light intensity of 320 µmol/m2×s)
Nominal and measured concentrations:
nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
Reference substance (positive control):
not specified
Species:
other: Nicotiana tabacum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 0.25 - < 1.25 other: mmol/kg
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Details on results:
EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
no data availble
Validity criteria fulfilled:
not specified
Conclusions:
Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
Executive summary:

The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.

According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.

The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Refer to the Analogue Approach Justification document provided in Section 13.
Reason / purpose for cross-reference:
read-across source
Species:
other: Nicotiana tabacum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 0.25 - < 1.25 other: mmol/kg
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: equivalent to 84 mg/kg soil
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable, well documented study
Qualifier:
no guideline followed
Principles of method if other than guideline:
seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Species:
Phaseolus vulgaris
Plant group:
Dicotyledonae (dicots)
Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
other: seedlings grown in polystyrene squares
Limit test:
no
Total exposure duration:
14 d
Species:
Phaseolus vulgaris
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 58.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Validity criteria fulfilled:
not specified
Conclusions:
In a 14 day eposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
Executive summary:

In a 14 day eposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.

Description of key information

NOEC (21 d): 84 mg/kg soil

Key value for chemical safety assessment

Additional information

Since no studies investigating the toxicity trisodium hydrogen EDTA (CAS 150-38-9) to terrestrial plants are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to a structurally related EDTA species was conducted. This read-across is justified in detail in the overall summary (IUCLID Section 6.3) and within the analogue justification in IUCLID Section 13.

According to Evangelou et al. (2006) disodium dihydrogen EDTA (CAS 139-33-3) showed adverse effects on growth of Nicotiana tabacum. The study was performed according to OECD 227. Seedlings of tobacco plants were exposed for 21 d in natural soil and the dry weight was analysed. Even though no NOEC was derived, the dry weight of plants strongly decreased at a concentration of 1.25 mmol/kg. Thus, the next lower concentration can be considered as NOEC value (0.25 mmol/kg equivalent to 84 mg/kg soil).