Methyltriphenylphosphonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan 04 - May 18, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyltriphenylphosphonium chloride

EC Number:

418-400-2

EC Name:

Methyltriphenylphosphonium chloride

Cas Number:

1031-15-8

Molecular formula:

C19H18ClP

IUPAC Name:

methyltriphenylphosphanium chloride

Study design

Inoculum or test system:

other: effluent from sewage plant

Details on inoculum:

Secondary effluent from a sewage plant was collected on the day of initiation of the test period. The sample was filtered through a paper filter, the first 200 ml were discarded. The remainder of the fIltrate was diluted 1:100 with physiological salt solution and kept under aerobic conditions at room temperature. The total count of viable cells in the fIltrate was in the order of magnitude of 1()6eFU/ml (colony forming units).

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

1.2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The test was performed in an air-conditioned test room to maintain a constant temperature of 20 +/- 1°C. However, this range of temperature was exceeded on day 0, the minimum and maximum average (two test bottles) temperatures for all series being 21.2/21.3°C. Since the deviation from the recommended temperature range was slight and all series were affected in a similar manner, the observed discrepancy would seem unlikely to influence the validity of the results.
Test series:
C: control: dilution water, mineral nutrient solutions without inoculum
B: inoculation blank
dilution water, mineral nutrient solutions and 50 uIll of inoculum.
R: reference: dilution water, mineral nutrient solutions, 50 ul/1 of inoculum and 2 ml of the reference solution (sodium benzoate, 1 g/l) => test concentration 2 mg/l
T: sample: 20 ml of the test article stock solution, mineral nutrient solutions as well as 50 ul of inoculum. => test concentration 1.2 mg/l

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

21.5

Sampling time:

28 d

Details on results:

The chemical oxygen demand of the reference substance sodium benzoate and the test article was determined experimentally according to IBR SOP/91/X/04 based on photometric analysis of COD using COD test vials (0-1500 mg COD/mg, HACH).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The percentage biodegradation of the test substance was 21.5% at the end of the test under the conditions of the study, so the test substance cannot be considered to be readily biodegradable.