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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well documented guideline study in compliance with GLP
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
5 ml of acetonitrile was added to 5 ml of water sample (control sample, sample contaminated with test material or fortified with standard solution) in Erlenmeyer flask. Contents of the flask was mixed throughly and applied to the chromatographic column in volume of 20 µL. Solutions of Fe(III)IDHA in concentration below 2 mg/L were injected to the chromatographic column without dissolving in acetone.
Vehicle:
no
Details on test solutions:
In the study test material was used in one concentration (limit test) - 100 mg/L. Concentration of test material was prepared by weighing of 10 mg of test material, precise mixing in 100 ml of Elendt water used in test, and then introducing to the test system.
Test organisms (species):
Daphnia magna
Details on test organisms:
Animals Daphnia manga used in experiments were taken from own laboratory culture of Institute of Organic industry, Branch Pszczyna. In the experiments animals in good physiological condition (checked by test with refrence substance - potassium dichromate) up to 24 hours old were used.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
not reported
Hardness:
not reported
Test temperature:
22-23oC
pH:
control: 7.6- 7.77
tested solution: 8.64 - 8.51
Dissolved oxygen:
control: 8.79-8.67
tested solution: 8.64-8.51
Salinity:
not applicable
Nominal and measured concentrations:
nominal concentration: 100mg/L
measured concentration:
t0= 101.06 mg/L
t48h = 94.4 mg/L
Details on test conditions:
The test environmental and the dilution medium used for preparing the test material solution was standarized elendt water M7, the same like used in culture. The test was run in plastic test containers of 100 mL capacity. Before test start Elend water was aerated for 30 min. Test concentrations as well as control was set up in four replicates. The volume of the test material solution in every replicate was 25 mL. Five individuals of Daphnia manga were used per each replicate (i.e. 20 animals per concentration). Therefore there was 5 mL of test solution per each animal (acc. to OECD gudeline should be at least 2 mmL). The test was run for 48 hours in natural photopheriod. The test was run in static manner. Animals were not fed during the test and the temperature was maintained in range 22-23oC. At the begining of the test and after 48 h pH values and content of dissolved oxygen were measured, the same measurements were performed for fresh solutions (pH-Oxi-metr inoLAB Level 3, WTW Germany).
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
>= 100 mg/L
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Details on results:
All validity criteria were met:
- mortality and immobilization of tested animals in control was 0%
- concentration of dissolved oxygen was 8.67 mg/L after 48h
Results with reference substance (positive control):
Resultsts for K2Cr2O7:
EC50(24h) > 3.2 mg/L
EC50(48h) = 1.13 mg/L (0.93-1.37 mg/L)
Validity criteria fulfilled:
yes
Conclusions:
Median concentration causing 50% immobilization of Dapnia manga population (EC50) after 48h was above 100 mg/L, EC0 after 48h is above or equal 100 mh/L, and EC100 after 48H is above 100 mg/L.
Executive summary:

The toxicity assessment of Fe(III)IDHA was performed according to OECD Guideline 202 and test method C.2 and in compiance with GLP (OECD, 1997). The aim of the study was evaluation of acute toxicity to Daphnia manga exposed to a range of concentrations of the test material (determination of EC50, EC0 and EC100 in mg/L after 24h and 48h exposure).

The test material was used in only one concentration (limit test) - 100 mg/L. Because of no mortality was observed in tested Daphnia population the EC50/48h value is above 100 mg/L, EC0 value is equel or above 100 mg/L, and EC100 value is above 100 mg/L.

Description of key information

Median concentration causing 50% immobilization of Dapnia manga population (EC50)

after 48h was above 100 mg/L, EC0 after 48h is above or equal 100 mh/L, and EC100 after 48H is above 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information