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EC number: 420-920-1 | CAS number: 128446-35-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- .beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose
- IUPAC Name:
- .beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose
- Reference substance name:
- -
- EC Number:
- 420-920-1
- EC Name:
- -
- Cas Number:
- 128446-35-5
- Molecular formula:
- Hill formula: (C42H70-nO35)(C3H7O)n; n(mittel)=5,25
- IUPAC Name:
- 5,10,15,25-tetrakis(hydroxymethyl)-40,44,47,49-tetrakis(2-hydroxypropoxy)-20,30,35-tris[(2-hydroxypropoxy)methyl]-2,4,7,9,12,14,17,19,22,24,27,29,32,34-tetradecaoxaoctacyclo[31.2.2.2³,⁶.2⁸,¹¹.2¹³,¹⁶.2¹⁸,²¹.2²³,²⁶.2²⁸,³¹]nonatetracontane-36,37,38,39,41,42,43,45,46,48-decol
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): hydroxypropylated .beta.-cyclodextrin
- Physical state: White powder
- Analytical purity: >95%
- Lot/batch No.: 74B008
- Expiration date of the lot/batch: 31 December 2002
- Stability in water: At least 96 h
- Stability under storage conditions: Stable
- Storage condition of test material: At room temperature in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: the accumulated biological mass produced in the treatment of waste water by the growth of bacteria and other micro-organisms in the presence of dissolved oxygen.
- Method of cultivation: no data
- Preparation of inoculum for exposure: sludge was coarsely sieved, washed and diluted with tap-water. After dilution a small amount of the
sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.7g/l of sludge, as used for the test). Before use the pH was checked.
- Pretreatment: The batch of sludge was used on subsequent days (maximum four days), therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of each working day. The sludge was kept aerated at test temperature until use.
- Initial biomass concentration: no data
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
Test conditions
- Hardness:
- no data available
- Test temperature:
- 19°C
- pH:
- 7
- Dissolved oxygen:
- no data available
- Salinity:
- no data available
- Nominal and measured concentrations:
- 3.2, 10, 32 and 100 mg/l (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- one at the start and one at the end
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The EC50 of 3,5-dichlorophenol was 15 mg/I (regression line:Y = 71.01 X -33.25, Y =·% inhibition of respiration and X = log concentration (mg/I)).
Since all criteria for acceptability of the test were met, this study was considered to be valid. - Results with reference substance (positive control):
- The mean respiration rate of control 1 and 2 was 38 mg O2/I/hr.The difference between the controls was 0%.
The mean respiration rate of control 2 and 3 was 41 mg O2/I/hr. The difference between the controls was 14.6%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, under the conditions of this present test, hydroxypropylated .beta.-cyclodextrin was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
- Executive summary:
The influence of hydroxypropylated .beta.-cyclodextrinon the respiration rate of activated sludge was investigated after a contact time of 30 minutes.
The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L133, adopted May 30, 1988.
Hydroxypropylated .beta.-cyclodextrinis white powder that was easily soluble in milli-Q water. A stock solution was prepared in milli-Q water at a concentration of 0.5 g/l. The pH of the clear stock solution was 7.6. A concentration of 100 mg/I, corresponding with 100 ml of the 0.5 g/l stock solution in 500 ml final volume, was tested in duplicate.
No significant inhibition in respiration rate of the sludge was recorded at 100 mg hydroxypropylated .beta.-cyclodextrinper litre. The duplicate measurement confirmed the result of the first measurement. Values of -7% and -5% inhibition were recorded (negative values indicate stimulation in respiration rate of the sludge). Hence, the EC50 for hydroxypropylated .beta.-cyclodextrinexceeded 100 mg/l and no further testing was necessary.
The respiration rates of the controls were within 15% of each other. The EC50 of the reference substance, 3,5 -dichlorophenol, was 15 mg/l. Therefore, the test was considered to be valid.
In conclusion, under the conditions of this present test,hydroxypropylated .beta.-cyclodextrinwas not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
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