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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 Mar - 25 Mar 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (no purity, no information on dissolved oxygen at test end)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted 1984
Deviations:
yes
Remarks:
dissolved oxygen at test end is not reported
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion of 2 g test material in 2 L of reconstituted water to give a stock solution from which dilutions were made to obtain the test series.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
270 mg/L as CaCO3
Test temperature:
21 °C
pH:
7.8 ± 0.2
Dissolved oxygen:
Medium was air-saturated at test start.
Nominal and measured concentrations:
Nominal: 0, 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 200 mL
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was prepared from the following mineral salt stock solutions that were diluted 1:4 in deionised water: 11.76 g/L CaCl2*2H2O, 4.93 g/L MgSO4*7H2O, 2.59 g/L NaHCO3, 0.23 g/L KCl. The medium was aerated until oxygen saturation was reached, pH was adjusted to 7.8 ± 0.2 with NaOH or HCl if necessary.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility was observed after 24 h and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes
- Results used to determine the conditions for the definitive study: The range finding study indicated a 48 h NOEC and a 48 h EC50 would be obtained within the range of the concentrations chosen.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL = 170 - 250 mg/L
Details on results:
- Mortalities in the control vessels: None
- The results of the test solution analysis showed an increasing carbon concentration with increasing nominal test concentration with the exception of the 10 mg/L test group. This erroneous value was considered to be due to sampling error and/or analytical variation. Also there was no significant change in carbon concentration observed over 48 h exposures period. The results were taken to demonstrate the stability of the test concentrations employed during the period of exposure.

Any other information on results incl. tables

Table 1: Additional information on effect concentrations

Duration

Endpoint

Effect conc.

Nom./Meas.

Conc. based on

Basis for effect

Remarks

24 h

EC50

330 mg/L

nominal

test mat.

mobility

95% CL = 270 – 410 mg/L

24 h

NOEC

56mg/L

nominal

test mat.

mobility

 

48 h

NOEC

56 mg/L

nominal

test mat.

mobility

 

Table 2: Cumulative Immobilisation Data for D. magna Exposed for 48 h to the test substance (R = Replicate)

Nominal Concentration of test substance (mg/L)

Cumulative immobilised Daphnia

(initial population: 10 per replicate)

24 h

48 h

R1

R2

Total

%

R1

R2

Total

%

Control

0

0

0

0

0

0

0

0

10

0

0

0

0

0

0

0

0

18

0

0

0

0

0

0

0

0

32

0

0

0

0

0

0

0

0

56

0

0

0

0

0

0

0

0

100

1

1

2

10

1

2

3

15

180

2

2

4

20

5

4

9

45

320

3

4

7

35

6

7

13

65

560

8

7

15

75

10

10

20

100

1000

10

10

20

100

10

10

20

100

Table 3: Results from Total Organic Carbon (TOC) Analysis

Samples

Nominal Concentration (mg/L)

Concentration of TOC corrected for control (mg C/L)

0 h

10

17.576

32

15.296

100

48.266

320

157.746

1000

518.246

48 h

10

11.951

32

13.181

100

43.841

320

147.851

1000

490.051

Applicant's summary and conclusion