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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Peer reviewed publication, cited by government and other agency reviews
Work is based on Vandium IV, using vanadium oxide. Considered to be suitable source of vanadium for purposes of this assessment.
The same research also examined chromosome aberration in human cells (separate IUCLID entry)

Data source

Reference
Reference Type:
publication
Title:
Genotoxic effects of vanadium (IV) in human peripheral blood cells
Author:
Rodriguez-Mercado J J, et al.
Year:
2003
Bibliographic source:
Toxicology letters, 144, 359-69 (2003)

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 479 (Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells)
GLP compliance:
not specified
Type of assay:
sister chromatid exchange assay in mammalian cells

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Work is based on Vandium IV, using vanadium dioxide. Considered to be suitable source of vanadium for purposes of this assessment.
Solublity of the vanadium dioxide is sufficiently high to ensure vanadium is present in soluble form.

Method

Species / strain
Species / strain / cell type:
lymphocytes:
Details on mammalian cell type (if applicable):
Human
Metabolic activation:
without
Test concentrations with justification for top dose:
2, 4, 8 or 16 µg/ml
Cytotoxicity at highest concentrations
Vehicle / solvent:
Water
Controls
Untreated negative controls:
yes
Remarks:
Water
Negative solvent / vehicle controls:
no
True negative controls:
no
Positive controls:
no

Results and discussion

Test results
Species / strain:
lymphocytes: Human
Metabolic activation:
without
Genotoxicity:
ambiguous
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
not applicable

Applicant's summary and conclusion

Conclusions:
Concluded as equivocal without metabolic activation.
Toxicity was seen.