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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Initial mortality and vigour test of 28 days
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
This report is part of wider research, including a reproductive toxicity test on fish.
A preliminary assessment was made at 28 days, stating with week-old fry
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
Principles of method if other than guideline:
One week old fry were exosed to different concentrations of vanadium for 28 days.
Low-dose groups were maintained for longer periods up to maturity and reproduction effects examined.
GLP compliance:
no
Specific details on test material used for the study:
Source of vanadium is described as predominantly sulphate, but oxy-sulphates and other salts will exist in the water column tested.
Results are expressed as vanadium.
Different valencies will also exist depending on pH
The report makes it clear that the vanadium form will be difficult to monitor and will change under conditions of analysis.
It is assumed that the vanadium under the test conditions would be penta-valent at the pH of the assessment
Analytical monitoring:
yes
Details on sampling:
Assessment for vanadium content
Vehicle:
no
Test organisms (species):
Jordanella floridae
Details on test organisms:
Common name 'Flagfish', chosen for short life cycle
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d
Remarks on exposure duration:
Lower dose treatment groups had extended exposure period of about 100 days to examine long-term effects including reproduction
Hardness:
Average 347 mg/l CaCO3
Test temperature:
Average 25 C
pH:
Average 8.15
Dissolved oxygen:
Average 7.4 mg/l
Nominal and measured concentrations:
0.051, 0.58, 3.4, and 6.3 mg/l of vanadium, nominal
(Measured 0, 0.051, 0.58, 3.4, and 6.3 mg/l)
Reference substance (positive control):
no
Duration:
28 d
Dose descriptor:
LC10
Effect conc.:
> 0.56 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Vanadium
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
LC10
Effect conc.:
> 1.9 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Starting with LC10 of 0.56 for V and using molecular weight of constituent with greatest proportion of vanadium
Details on results:
There was 100% mortality at 5.6 mg/l vanadium and > 50% mortality at 3.14 mg/l.
The lower concentrations of 0, 0.056 and 5.6 had a level of 5 - 10% mortality, including 4/60 deaths in the control.
Conclusions:
It is concluded that the oxysuphate salts will be toxic at over 2 mg/l (0.56 mg/l vanadium).

Longer term exposure of 70 days showed good survival at 0.48 mg/l vanadiaum suggesting that there is no accumulative toxicity.
There was good survival, good egg production and good viablity of fry at 0.48 mg/l in the reproduction group extended to over 90 days exposure.

Description of key information

It is concluded that the oxysulphate salts will be toxic at over 2 mg/l (0.56 mg/l vanadium) and as vanadium is an essential element at low concentrations, it is not considered accumulative, suggesting that classification is not required

Longer term exposure of 70 days showed good survival at 0.48 mg/l vanadiaum suggesting that there is no accumulative toxicity.

There was good survival, good egg production and good viablity of fry at 0.48 mg/l in the reproduction group extended to over 90 days exposure.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
2 mg/L

Additional information