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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1984
Deviations:
yes
Remarks:
Because of the limited stability of the test substance the exposure period was 24 hours instead of 48 hours. This is in accordance with the EEC guidelines mentioned in the protocol
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised
EC Number:
402-370-2
EC Name:
(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised
Cas Number:
149057-70-5
Molecular formula:
C14H28AlNO6
IUPAC Name:
octaaluminium(3+) octakis((2Z)-4-ethoxy-4-oxobut-2-en-2-olate) octakis(1-methoxypropan-2-olate) octakis(2-(dimethylamino)ethan-1-olate)
Test material form:
liquid
Details on test material:
purity not specified, batch 25/2/87R

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The samples taken from the test media (approx. 100 ml) were treated as follows:
10 ml of a sample was led through an activated Sep C18 column followed by eluation with 1 ml methanol (Merck, lichrosolv), The 100 µl eluent was diluted with methanol and internal standard in such a way that the response of the gas chromatograph was comparable with that recorded for a calibration standard of concentrations approximately of 250 mg/L.

Test solutions

Vehicle:
yes
Details on test solutions:
Dutch Standard Water (DSW)

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Water media type:
other: Dutch Standard Water (DSW)
Total exposure duration:
24 h

Test conditions

Hardness:
11.7 DH
pH:
8.2 +/- 0.2
Nominal and measured concentrations:
nominal: 320, 560, 1000 mg/L
actual after 0h: 100, 145, 230 mg/L
Reference substance (positive control):
yes
Remarks:
K2Cr207 {Merck-Darmstadt, Germany) was used as a reference substance at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/1.

Results and discussion

Effect concentrations
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Conclusions:
The present study reveals that A6/1/0 is toxic to Daphnia magna at nominal concentrations higher than 320 mg/1 in a 24h immobility test according to EEC method C2.
Executive summary:

The present study reveals that A6/1/0 is toxic to Daphnia magna at nominal concentrations higher than 320 mg/L in a 24h immobility test according to EEC method C2.