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EC number: 806-543-7 | CAS number: 215917-77-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Test guideline under Japan Chemical Substances Control Law
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Rangefinder:
No samples were taken for analysis. A preliminary solubility trial was conducted prior to the range finder, which included prolonged stirring (48 hours), followed by filtration (Millex 0.45 µm) of a 100 mg/L stock (in triplicate). This provided a mean solubility of 6.8 mg/L, which was used as the limit concentration in the definitive test.
Definitive:
- Concentration: 6.8 mg/L
- Frequency: Samples for analysis were taken from the limit test concentration, control and solvent control at the start of the study (t = 0h Fresh), (t = 48h Expired), (t = 48h Fresh) and (t = 96h Expired). This provided two sets of fresh and expired samples.
- Sampling method: 0.75 mL of medium used. No detail of where this was removed but assumed from the centre of the tanks.
- Sample storage conditions before analysis: Not detailed, but assumed to be analysed on the day of sampling. - Vehicle:
- yes
- Details on test solutions:
- Preparations of test medium started with the preparation of a solvent stock solution in N,N-Dimethylformamide with the test material. An aliquot of this was then added into a media bottle containing test medium and stirred for 15 minutes (magnetically) which provided the limit concentration of 6.8 mg/L. This was then transferred to a 5 L glass aquaria and mixed with a glass rod. An equivalent amount of solvent was added for the solvent control (vehicle) group. All prepared test solutions were clear and colourless.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Medaka
- Source: Kitamura-yourijou (388 Gunchiku 12-bancho, Yatsushiro, Kumamoto, Japan).
- Length at study initiation: 2.39 cm (Range 2.13 - 2.72 cm, n = 10)
- Weight at study initiation: 0.131 g (Range 0.101 - 0.177 g, n = 10)
(Weight/length values measured for the control fish at the end of the testing period (96 hours)).
- Feeding during test: no feeding for 24 hours prior to test and during test period.
ACCLIMATION
- Acclimation period: at least 14 days
- Feeding frequency: daily with Tetramin and/or Tetrawerke
- Health during acclimation (any mortality observed): < 5% mortality during 7 days prior to start of test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Fish assumed to be euthanised after the test (96 hours). No detail provided.
- Hardness:
- 57 mgCaCO3/L (Most recently available diluent water analysis).
- Test temperature:
- 23.8 to 24.3 °C
- pH:
- 7.2 to 7.6
- Dissolved oxygen:
- 7.8 to 8.5 mg/L
- Nominal and measured concentrations:
- The nominal concentration was 6.8 mg/L.
The measured concentrations were 93% at 0 hours (Fresh), 90% at 48 hours (expired), 96% at 48 hours (Fresh) and 97% at 96 hours (Expired), providing a time weighted mean value of 94% of nominal (6.39 mg/L measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 5 L (Glass)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.0 g fish/ litre or less
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated water processed by treating tap water with an activated charcoal filter, and sodium thiosulphate
- Test medium: Dechlorinated water with the following composition:
Ca2+ 16 mg/L
Mg2+ 4.3 mg/L
Na+ 7.0 mg/L
K+ 1.0 mg/L
Cl- < 0.1 mg/L
HCO3(-) 57 mg/L
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark, daily (1000 Lux or less)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and effect observations at 3, 24, 48, 72 & 96 hrs following the start of exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test 6.8 mg/L (limit of solubility in test medium)
- Justification for using less concentrations than requested by guideline: no toxicity at limit concentration
- Range finding study:
Test concentrations: 0.068, 0.68 and 6.8 mg/L
- Results used to determine the conditions for the definitive study: no toxicity observed - Reference substance (positive control):
- yes
- Remarks:
- Aqueous Copper Sulphate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 6.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Greater than limit of solubility
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality or sublethal effects were noted at any point in either control, solvent control or test vessels in the main study
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- LC50: 0.62 mg/L (confidence limit: 0.42 - 0.89 mg/L). Within historical range. - Sublethal observations / clinical signs:
Exposure Period (hour) LC50 (mg/L) 95% confidence limit (mg/L) Statistical method 24 >6.39 - - 48 >6.39 - - 72 >6.39 - - 96 >6.39 - - NOEC = 6.39 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In a 96 hr acute toxicity study, Medaka (Oryzias Latipes) were exposed to the test substance at nominal concentrations of 0 (control), 0 (solvent control) and 6.39 mg/L under semi-static conditions (measured concentrations). Due to the low solubility of the test substance in the test medium, concentration levels that might be toxic for fish could not be reached. The 96hr LC50 was therefore above the solubility limit of the test substance in test medium (> 6.39 mg/L).
The NOEC value, based on mortality/sublethal effects, was 6.39 mg/L.
The limit test concentration was determined to be the limit of solubility in the test media, based on the results of a preliminary solubility trial.
This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute fish toxicity study.
Reference
Description of key information
In a 96 hr acute toxicity study, Medaka (Oryzias Latipes) were exposed to the test substance at measured concentrations of 0 (control), 0 (solvent control) or 6.93 mg/L under semi-static conditions. The NOEC value, based on mortality/ sublethal effects, was 6.93 mg/L.
Key value for chemical safety assessment
Additional information
Due to the low solubility of the test substance in test medium, concentration levels that might be toxic for fish could not be reached.The 96hr LC50 was above the solubility limit of the test substance in test medium (> 6.93 mg/L).
The NOEC value, based on mortality/ sublethal effects, was 6.93 mg/L.
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