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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: Test guideline under Japan Chemical Substances Control Law
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Lot No.: TFu12268
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Mixed Liquor Suspended Solids (MLSS): 4300 mg/L
Source: Chemical Evaluation and Research Institute, Japan
Date of Receipt: 2013-06-21
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
test mat. analysis
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Temperature: 25 ± 1°C
Test volume: 300 mL

Test bottle contents :
Bottle 1 : Aniline + activated sludge + basal medium (Positive control)
Bottle 2 : Activated sludge + basal medium (Inoculum blank)
Bottles 3-5 : Test substance + activated sludge + basal medium
Bottle 6 : Test substance + ultrapure water (Abiotic sterile control)
Reference substance:
aniline
Test performance:
From the biodegradability result based on BOD (average 0%) and that of the residual test item remaining (average 0%), it is concluded that the test substance is not readily biodegradable.
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Key result
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
28 d
Details on results:
-Degradability based on BOD
The degree of degradability based on the BOD measurements was 0, 1, and 0% for bottles 3, 4, and 5.
-Degradability based on DOC
Degradability was not calculated because the test substance was insoluble in water.
-Degradability based on the residual test substance concentration
The degree of degradability based on the residual test substance concentration was 0, 0, and 1 % for bottles 3, 4 and 5, respectively.
Results with reference substance:
The degradability of aniline, based on the BOD measurements was 52.8 % after 7 days. The activity of the sludge was thus shown to be satisfactory.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
It is concluded that the test substance was not readily biodegradable and no transformation products were generated under the conditions of the modified MITI test.

Description of key information

A modified MITI test was conducted to assess the Ready Biodegradability of the test substance.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

From the degradability results based on the BOD and the residual test substance concentration, it is concluded that the test substance was not readily biodegradable and was not transformed under the conditions of the Modified MITI test.