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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 Nov - 06 Dec 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: Circular on Test Methods of New Chemical Substances (Japan), biodegraadation test
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetradecyl myristate
EC Number:
221-787-9
EC Name:
Tetradecyl myristate
Cas Number:
3234-85-3
Molecular formula:
C28H56O2
IUPAC Name:
tetradecyl myristate

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment
Details on inoculum:
- Source of inoculum/activated sludge (location, sampling depth): location: 10 sites (4 sewage plants, 3 rivers, 1 lake and 2 bays) around Japan, sampling depth: return sludge in sewage plants, surface water and surface soil of foreshore
- Laboratory culture: Thirty minutes after stopping of aeration, discard about one third of the whole volume of supernatant and add an equal volume of a solution (pH adapted to 7.0 with NaOH) containing 0.1% each of glucose, peptone and potasium orthophosphate, to the settled material and re-commence aeration. This procedure was repeated once per day.
- Temperature: 25 ± 2 °C
- Storage conditions: appearance and general condition of sludge were observed with a light microscope, sediments, pH, temperature and dissolved oxygen
- Storage length: started to use: 15 Oct 1991
- Concentration of sludge: 5500 mg/L
- Water filtered: no
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
TEST CONDITIONS
- Composition of medium: recommended medium in the guideline
- Test temperature: 25 ± 1 °C
- Suspended solids concentration: 30 mg/L
- Continuous darkness: no data

TEST SYSTEM
- Culturing apparatus: closed oxygen consumption measuring machine
- Number of culture flasks/concentration: 1 (water + test substance), 3 (sludge + test sub.), 1 (sludge + reference sub.), 1 (sludge)
- Measuring equipment: Coulometer
- Details of trap for CO2 and volatile organics if used: soda lime

SAMPLING
- Sampling frequency: continuously until day 28
- Sterility check if applicable: no
- Sample storage before analysis: no data

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
67
Sampling time:
28 d
Remarks on result:
other: BOD/ThOD
Parameter:
% degradation (test mat. analysis)
Value:
94
Sampling time:
28 d
Details on results:
No 10-day window required. Substance was readily biodegradable.

BOD5 / COD results

Results with reference substance:
The oxygen consumption for aniline in the above used test system reached 58% and 75% degradation after 7 days and 14 days.

Any other information on results incl. tables

Table 1. Results of analysis for BOD, DOC and GC

 

water + test sub.

sludge + test sub.

Theoretical amount

sample No. 6

sample No. 3

sample No. 4

sample No. 5

BOD

mg

0

60.8

64.5

60.6

92.7

DOC

mgC

-

0.8

1.1

1.2

23.8

% (*1)

-

3

5

5

-

GC of C9H14O6

mg

30.4

2.1

0.0

3.0

30

% (*1)

101

7

0

10

-

(*1): Residue percentage = Residue amount (mg) / Theoretical amount (mg) x 100

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable