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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
30 October 2018 - 27 November 2018
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Test method according to OECD 301F. GLP study.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test).
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L
- Sampling method: DOC analysis using spectrophotometer Hach DR 3900 and Hach-Lange reagents.
- Sample storage conditions before analysis: samples were analysed immediately after sampling.


Vehicle:
no
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Source: aeration tank of Sewage Treatment Plant ”Czajka” , Warsaw, receiving predominantly domestic sewage.
- Name and location of sewage treatment plant where inoculum was collected: "Czajka", Warsaw.
- Preparation of inoculum for exposure: The coarse particles were removed by settling and the supernatant was discarded. The sludge was washed in the mineral medium. The concentrated sludge was suspended in mineral medium to yield a concentration of 3-5 g suspended solids/l and it was aerated, at the test temperature of 22ºC, until application next day. A sample was withdrawn just before use for the de termination of the dry weight of the suspended solids.
- Pretreatment: Inocula was pre-conditioned to the experimental conditions. Pre-conditioning consisted of aerating activated sludge in mineral medium during 1 day at the test temperature of 22 ºC.
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Test temperature:
22 ± 2°C
pH:
7.33-7.51
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: respirometer flasks.
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, volume of test solution in flask, V: 0.164 L
- No. of vessels per concentration (replicates): 3, each containing 100 mg/L test item and 30 mg/L SS inoculum.
- No. of vessels per control (replicates):
Inoculum blank: 3 replicates, each containing 30 mg/L SS inoculum.
Procedure control: 3 replicates, each containing 100 mg/L reference item (sodium acetate) and 30 mg/L SS inoculum.
Toxicity control: 3 replicates, each containing 100 mg/L test item, 100 mg/L reference item (sodium acetate) and 30 mg/L SS inoculum.
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 10ml of solution (A) in 800 ml water, plus 1 ml solutions (B), (C), (D) and dilution water up to 1 L. The following stock solutions were used, prepared with analytical grade reagents: Solution (A) contains: 8.50 g monopotassium dihydrogen orthophosphate (KH2PO4), 21.75 g dipotassium monohydrogen orthophosphate (K2HPO4), 33.40 g disodium monohydrogen orthophosphate dihydrate (Na2HPO4·2H2O), 0.50 g ammonium chloride (NH4Cl), in 1 L water. Solution (B) contains: 27.50 g calcium chloride, anhidrous (CaCl2) in 1L water. Solution (C) contains: 22.50 g magnesium sulphate heptahydrate (MgSO4·7H2O) in 1L water. Solution (D) contains: 0.25 g iron(III) chloride hexahydrate (FeCl3·6H2O) in 1L water.
The water used is double-distilled, containing 3.4±0.5 mg/L of organic carbon (< 10% of the organic carbon content introduced by the test item), checked by DOC analysis using spectrophotometer Hach DR 3900 and Hach-Lange reagents.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
yes
Remarks:
acetic acid, sodium salt CAS No: 127-09-3, purity p.a. 99.7%, source: CHEMPUR, Piekary Śląskie, Polska.
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (77.9% of biodegradation at 28 days) and did not inhibit the biodegradation in the toxicity test (41.8% of biodegradation after 14 days) up to 100 mg/L.

Results with reference substance (positive control):
- Results with reference substance valid: yes
- The reference item reached 88.2% of biodegradation and the level for ready biodegradability by 4 days (pass level: biodegradation higher than 60% before day 14)

Table 1. Sample oxygen uptake: biodegradability.

 

time, days

1

3

5

7

9

12

14

16

18

21

23

25

28

Test item O2uptake, mg/L

a1

5.6

46.4

87.2

114.9

136.9

159.9

172.5

182.7

191.4

207.4

219.7

231.1

245.8

a2

3.8

39.4

78.1

107.0

137.4

173.4

190.1

203.9

216.4

233.6

245.5

256.8

272.6

a3

5.3

39.5

69.0

99.8

132.1

176.9

198.5

218.6

234.6

255.9

268.2

276.3

286.8

am. avg

4.9

41.8

78.1

107.2

135.5

170.1

187.1

201.7

214.1

232.3

244.5

254.8

268.4

Blank test O2uptake mg/L

b1

7.0

14.1

16.3

20.3

21.3

23.4

24.2

27.4

28.0

30.6

31.8

32.9

33.3

b2

6.6

13.1

15.7

19.4

21.6

23.7

25.0

26.1

27.2

29.5

29.5

31.4

32.2

b3

6.6

13.1

16.4

17.6

19.9

22.7

24.1

26.2

27.4

31.1

32.1

32.5

36.0

bm. avg

6.7

13.4

16.1

19.1

20.9

23.3

24.4

26.6

27.6

30.4

31.1

32.3

33.9

Reference item O2uptake. mg/L

w1

18.3

56.4

68.2

78.1

83.5

87.8

90.1

93.5

93.8

97.2

99.6

100.8

103.7

w2

20.1

58.7

73.6

81.8

87.2

90.0

93.8

95.0

95.4

97.9

99.1

99.4

101.7

w3

21.2

59.5

72.2

79.3

84.6

89.6

91.0

93.8

95.2

98.2

98.5

101.1

103.1

wm. avg

19.9

58.2

71.3

79.7

85.1

89.1

91.6

94.1

94.8

97.7

99.1

100.5

102.8

Toxicity control O2uptake. mg/L

tox1

18.7

63.4

92.1

115.7

134.8

158.7

171.3

182.0

194.2

212.9

226.8

239.7

259.6

tox2

17.2

58.7

96.7

123.2

148.7

178.5

199.9

214.7

229.4

248.7

262.1

276.0

295.4

tox3

19.4

59.1

94.5

117.1

139.1

164.3

177.1

188.6

198.1

213.7

223.8

234.6

249.1

toxm.avg

19.1

61.3

93.3

116.4

136.9

161.5

174.2

185.3

196.1

213.3

225.3

237.1

254.4

Corrected

test item O2uptake, mg/L

(a1-bm)

-1.1

33.0

71.1

95.8

116.0

136.7

148.1

156.1

163.8

177.0

188.5

198.8

212.0

(a2-bm)

-2.9

26.0

62.0

87.9

116.5

150.1

165.7

177.3

188.8

203.2

214.4

224.5

238.7

(a3-bm)

-1.4

26.1

52.9

80.7

111.2

153.6

174.1

192.0

207.0

225.4

237.1

244.1

253.0

Reference item % degradation

ThOD = 0.78

mgO2/mg

C = 100 mg/L

R1(w1)

14.8

55.1

66.8

75.7

80.2

82.7

84.2

85.8

85.0

85.6

87.8

87.9

89.5

R2(w2)

17.1

58.0

73.7

80.3

85.0

85.6

88.9

87.7

87.0

86.5

87.1

86.1

86.9

R3(w3)

18.6

59.1

71.9

77.2

81.6

85.0

85.3

86.2

86.7

86.8

86.4

88.3

88.7

Rwavg

15.9

56.6

70.2

78.0

82.6

84.1

86.6

86.8

86.0

86.0

87.5

87.0

88.2

Test item% degradation

ThOD = 3.01

mgO2/mg

C = 100 mg/L

R1(a1)

0.0

11.0

23.6

31.8

38.5

45.4

49.2

51.9

54.4

58.8

62.6

66.1

70.4

R2(a2)

0.0

8.6

20.6

29.2

38.7

49.9

55.0

58.9

62.7

67.5

71.2

74.6

79.3

R3(a3)

0.0

8.7

17.6

26.8

36.9

51.0

57.8

63.8

68.8

74.9

78.8

81.1

84.0

Raavg

0.0

9.4

20.6

29.3

38.1

48.8

54.0

58.2

62.0

67.1

70.9

73.9

77.9

Toxicity test

% degradation

 

R1(tox1)

3.2

13.2

20.0

25.5

30.0

35.7

38.7

41.0

44.0

48.2

51.6

54.7

59.6

R2(tox2)

2.8

11.9

21.3

27.5

33.7

41.0

46.3

49.6

53.2

57.6

60.9

64.3

69.0

R3(tox3)

3.4

12.0

20.7

25.9

31.2

37.2

40.3

42.8

45.0

48.3

50.8

53.4

56.8

Rtoxavg

3.1

12.4

20.7

26.3

31.6

38.0

41.8

44.5

47.4

51.4

54.5

57.5

61.8

Table 2. The pH values of test flasks (no adjustment of pH was conducted).

flask #

7

8

9

1

2

3

4

5

6

10

11

12

Test item

Inoculum blank

Reference item

Toxicity test

initial

7.50

7.51

7.50

7.32

7.48

7.23

7.29

7.34

7.39

7.50

7.50

7.49

final

7.33

7.34

7.35

7.53

7.50

7.51

8.62

8.59

8.61

8.04

7.85

8.16

 

At 28 days of test performance:

- The test item attained 77.9% of biodegradation (>60% reached within the 28-day period of the test)

- The reference item reached 88.2 % of biodegradation and the level for ready biodegradability by 4 days (>60% reached before day 14)

- In the toxicity test the biodegradation was equal to 41.8% in 14 days (>25% in 14 days). Therefore, the test item is not inhibitory.

- The oxygen uptake of the inoculum blank was equal to 33.9 mg/l in 28 days (<60 mg/l in 28 days)

- The pH values of all flasks containing test item were in the range 7.33-7.51 (inside the range 6-8.5)

 

Validity criteria fulfilled:
yes
Remarks:
(difference of replicates <20%, reference item pass level (60%) on day 4, blank oxygen uptake 33.9 mgO2/l in 28 days, pH inside the range 6-8.5,test item oxygen consumption > 60%. Biodegradation (b. on ThOD) 41.8% in toxicity test: not inhibitory)
Conclusions:
The NOEC (28d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on no inhibitory effect observed in a biodegradation test).
Executive summary:

A ready biodegradability manometric respirometry test was perfomed according to OECD Guideline 301 F. The test item was incubated for 28 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. Additionally, to check the possible inhibitory effect of the test item, a toxicity test was run in parallel (100 mg/L test item with 100 mg/L reference substance sodium acetate and 30 mg/L SS activated sludge inoculum) at the same conditions. The NOEC (28d) for microorganism toxicity of the test item was determined to be 100 mg/L since the substance degraded well (77.9% of biodegradation at 28 days) and did not inhibit the biodegradation in the toxicity test (41.8% of biodegradation after 14 days) up to 100 mg/L.


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
23 July 1980 - 06 August 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
yes
Remarks:
(test duration lower than 28 d)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
14 d
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
not specified
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (73% of biodegradation at 14 days).
Validity criteria fulfilled:
not specified
Conclusions:
The NOEC (14d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed on dipentene. The test item was incubated for 14 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (14d) of the test item was determined to be 100 mg/L since the substance degraded well (73% of biodegradation at 14 days).


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
25 March 1997 - 22 April 1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Test method according to OECD guideline 301 C. GLP study.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
yes
Remarks:
(aniline)
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (72% of biodegradation at 28 days).
Results with reference substance (positive control):
- Results with reference substance valid: yes
Validity criteria fulfilled:
yes
Conclusions:
The NOEC (28d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed according to OECD Guideline 301 C. The test item was incubated for 28 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (28d) of the test item was determined to be 100 mg/L since the substance degraded well (72% of biodegradation at 28 days).


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
yes
Remarks:
(test duration lower than 28 d)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
2 wk
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
not specified
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (84.6% of biodegradation at 14 days).
Validity criteria fulfilled:
not specified
Conclusions:
The NOEC (14d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed on alpha terpineol. The test item was incubated for 14 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (14d) of the test item was determined to be 100 mg/L since the substance degraded well (84.6% of biodegradation at 14 days).


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
03 February 1988 - 02 March 1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Test method according to OECD guideline 301 C. GLP study.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
yes
Remarks:
(aniline)
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (94% of biodegradation at 28 days).
Results with reference substance (positive control):
- Results with reference substance valid: yes
Validity criteria fulfilled:
yes
Conclusions:
The NOEC (28d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed according to OECD Guideline 301 C. The test item was incubated for 28 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (28d) of the test item was determined to be 100 mg/L since the substance degraded well (94% of biodegradation at 28 days).


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
12 February 1987 - 27 February 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Test method according to OECD guideline 301 C. GLP study.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
yes
Remarks:
(test duration lower than 28 d)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: activated sludge was collected from 10 different locations, including sewage tratment plants and natural waters (river, ocean or lakes)
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
25 ± 1ºC
pH:
7
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: Closed oxygen consumption measurement device (Coulometer made by Okura Electric Co.)
- Type (delete if not applicable): closed
- Aeration: yes, magnetic stirrer
- No. of vessels per concentration (replicates): 3
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter: 6000 mg/L SS

OTHER TEST CONDITIONS
- Adjustment of pH: yes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
yes
Remarks:
(aniline)
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (88% of biodegradation at 14 days).
Results with reference substance (positive control):
- Results with reference substance valid: yes
- Relevant effect levels: The degrees of decomposition of aniline after 7 and 14 days obtained by BOD were 53% and 87%, respectively. Thus, it was confirmed that the test conditions of this test were effective.
Validity criteria fulfilled:
yes
Conclusions:
The NOEC (14d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed according to OECD Guideline 301 C. The test item was incubated for 14 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (14d) of the test item was determined to be 100 mg/L since the substance degraded well (88% of biodegradation at 14 days).


Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Test method according to OECD guideline 301 C. GLP study.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L



Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 30 mg/L SS
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Sludge concentration (weight of dry solids per volume): 30 mg/L.
- Nitrification inhibitor used (delete if not applicable): none.

OTHER TEST CONDITIONS
- Adjustment of pH: no.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biodegradation % based on oxygen consumption.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L
Reference substance (positive control):
not specified
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Readily biodegradability
Details on results:
The NOEC of the test item was determined to be 100 mg/L since the substance degrades well (90-95% of biodegradation at 28 days).
Validity criteria fulfilled:
yes
Conclusions:
The NOEC (28d) for microorganism toxicity of the test item was determined to be 100 mg/L (based on biodegradation).
Executive summary:

A ready biodegradability test was perfomed according to OECD Guideline 301 C. The test item was incubated for 28 days at a concentration of 100 mg/L with 30 mg/L SS activated sludge inoculum under aerobic conditions in a mineral medium. The NOEC (28d) of the test item was determined to be 100 mg/L since the substance degraded well (90 -95% of biodegradation at 28 days).


Description of key information

Key study. Test method according to OECD 301F, GLP study.The 28 day-NOEC for microorganism toxicity of the test item was determined to be 100 mg/L (based on no inhibitory effect observed in a biodegradation test).

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

Key study: A ready biodegradability manometric respirometry test was perfomed on the test item according to OECD Guideline 301F. Under the test conditions, the 28 day-NOEC for microorganism toxicity of the test item was determined to be 100 mg/L since the substance degraded well (77.9% of biodegradation at 28 days) and did not inhibit the biodegradation in the toxicity test (41.8% of biodegradation after 14 days) up to 100 mg/L.

Supporting studies: Data on toxicity to microorganisms of the substance's constituents are available from experimental data:

Alpha terpineol: Test method OECD Guideline 301C (MITI Japan, 1978). The NOEC (14d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).

Terpinolene: Test method OECD Guideline 301C (MITI Japan, 1997). The NOEC (28d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).

Gamma terpinene: Test method OECD Guideline 301C (MITI Japan, 1988). The NOEC (28d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).

Dipentene: Test method OECD Guideline 301C (MITI Japan, 1980). The NOEC (14d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).

Alpha pinene: Test method OECD Guideline 301C (MITI Japan, 2005). The NOEC (28d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).

p-cymene: Test method OECD Guideline 301C (MITI Japan, 1987). The NOEC (14d) was determined to be 100 mg/L (based on no inhibitory effect observed, readily biodegradable substance).