reaction product of: C.I. Leuco Sulfur Black 1 with (3-chloro-2-hydroxypropyl)trimethylammonium chloride

Short-term toxicity to fish

Description of key information

LC50 (96h, Oncorhynchus mykiss) = 4.6 mg/l (nominal)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.6 mg/L

Additional information

The toxicity of the test item to fish was evaluated in an experimental study on Rainbow trout (Oncorhynchus mykiss) according to the OECD Guideline 203 (1992) and the EU Method C.1 (1992). Young Rainbow trout were exposed to various concentrations of the test item in aqueous test media for 96 hours in a static test under defined conditions, and were monitored daily for mortality and symptoms of intoxication. The nominal concentrations of test item were 0.92, 2.0, 4.2, 9.2 and 20.0 mg/l. Aquaria were monitored for oxygen concentration, pH level, temperature and behaviour of the test item in test media.

The mean measured test substance concentrations were determined to be 90 % to 97 % of the nominal values (calculated as the average over all measurements per test concentration). Since these mean measured test substance concentrations were well above 80 % of the nominal values, the reported biological results are related to the nominal test substance concentrations.

Strong colouration was observed at all time points and at all concentrations, caused by the test item, therefore toxicity and symptoms were difficult to monitor. No mortality or toxicity was observed among the control group or test groups at concentrations of 0.92 and 2.0 mg/l test item, therefore the NOEC for 96 hours was determined to be 2.0 mg/l (LOEC = 4.2 mg/l). The LC50 was determined to be 4.6 at 96 hours (C.I. 95%: 3.0 - 7.0).