Hydroxyacetone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018/10/29 - 2018/11/02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, 55116 Mainz

Analytical monitoring:

yes

Details on sampling:

At the beginning and at the end of the test, the content of the test item in the test solution was determined using HPLC-UV-determination. The concentration determined at the start of the test was 96 % of the nominal concentration. At the end of the test the determined concentration was 95 % of the nominal concentration.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution containing 100.0 mg/L (resp. 92.4 μL/L based on a density of 1.0828 ± 0.0002 g/cm3 at 20.0 ± 0.2 °C, determined in LAUS study 17100906G912) test item in dilution water was prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin
- Age of parental stock: 0 - 24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.502 mmol/L

Test temperature:

20.5 - 21.2 °C

pH:

7.6 - 7.8

Dissolved oxygen:

8.4 - 9.3 mg/L

Nominal and measured concentrations:

nominal: 100 mg/L; measured: 95.76 mg/L (0 h), 95.26 mg/L (48 h)

Details on test conditions:

TEST SYSTEM
- Test vessel: Closed glass flasks
- Type: closed
- Material, size, headspace, fill volume: nominal volume 65 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Daphnia medium
CaCl2*2 H2O = 293.80 mg/L
MgSO4*7H2O = 123.30 mg/L
NaHCO3 = 64.80 mg/L
KCl = 5.80 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 16/8 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
EC50 (24 h), EC50 (48 h), NOEC (48 h), LOEC (48 h)

RANGE-FINDING STUDY
The concentration of 100 mg/L to be tested is based on the result of a non-GLP pre-test.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In the blank control and the treatment none of the Daphnia was immobilised.

Results with reference substance (positive control):

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201714R201). The 24h-EC50 value based on immobility was determined as 1.9 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as specified in the OECD guideline. Since the fits showed sufficient statistical correspondence of the data with the dose-response-equation, this reference study can be considered valid.

Validity

-Immobilisation in the controls may not exceed 10 %.

Immobilisation in the controls was 0 %.

-The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L.

The lowest concentration of dissolved oxygen at the end of the test was 8.4 mg/L. Consequently all validity criteria are met and the study is considered valid.

Validity criteria fulfilled:

yes

Remarks:

please refer to "Any other information on results"

Conclusions:

The following results were determined for the test item Hydroxyacetone (species: Daphnia magna).
48h-NOEC >= 100 mg/L
48h-LOEC > 100 mg/L
24h-EC50 > 100 mg/L
48h-EC50 > 100 mg/L

Executive summary:

One valid experiment according to OECD Guideline 202 and GLP was performed. The study was conducted as a limit test at 100 mg/L. To avoid volatilization of the test item, the study was performed in closed vessels. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted. None of the animals was immobilised in the blank control. The test concentration showed no toxicity / immobilisation. No other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water was noted either. Therefore no statistical evaluation was performed. Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable. At the beginning and at the end of the test, the content of the test item in the test solution was determined using HPLC-UV -determination. The concentrations determined at the start of the test were 96 % of the nominal concentration. At the end of the test the determined concentrations were 95 % of the nominal concentration. Therefore, the determination of the biological results is based on the nominal concentration. The following results were determined for the test item Hydroxyacetone (species: Daphnia magna):

48h-NOEC >= 100 mg/L

48h-LOEC > 100 mg/L

24h-EC50 > 100 mg/L

48h-EC50 > 100 mg/L

Description of key information

D. magna, static, 48 h, OECD 202, GLP, EC50 > 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

One valid experiment according to OECD Guideline 202 and GLP was performed. The study was conducted as a limit test at 100 mg/L. To avoid volatilization of the test item, the study was performed in closed vessels. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted. None of the animals was immobilised in the blank control. The test concentration showed no toxicity / immobilisation. No other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water was noted either. Therefore no statistical evaluation was performed. Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable. At the beginning and at the end of the test, the content of the test item in the test solution was determined using HPLC-UV -determination. The concentrations determined at the start of the test were 96 % of the nominal concentration. At the end of the test the determined concentrations were 95 % of the nominal concentration. Therefore, the determination of the biological results is based on the nominal concentration. The following results were determined for the test item Hydroxyacetone (species: Daphnia magna).

48h-NOEC >= 100 mg/L

48h-LOEC > 100 mg/L

24h-EC50 > 100 mg/L

48h-EC50 > 100 mg/L