Hydroxyacetone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018/10/29-2018/11/02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, 55116 Mainz (15.05.18)

Specific details on test material used for the study:

stored dark and dry at room temperature (20 ± 5°C)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sampling method: Observations were made every 24 hours, measuring pH and O2-concentration of the test solution in each vessel and documenting mortalities. The content of the test item in the test vessels was measured at the beginning and at the end of the test and at the medium renewal after 48 hours in the fresh and the old solutions. In the control, the content of test item was measured only at the beginning and at the end of the test.
- Sample storage conditions before analysis: Samples were stored together with the QS sample for 3 days

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A test solution containing 100 mg/L in tap water was prepared, homogenized by stirring
- Controls: tap water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Danio rerio
- Source: Dehner Garten Center, 67433 Neustadt
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): length 2 ± 1 cm

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): test conditions
- Type and amount of food during acclimation: warmwater fishfood
- Feeding frequency during acclimation: 3 times a day
- Health during acclimation (any mortality observed): During 7 days mortality < 5 % was observed

FEEDING DURING TEST
none

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

0.71 mmol/L (analysis: March 2017)

Test temperature:

23.5-24.0 °C

pH:

7.8-8.0

Dissolved oxygen:

5.1-8.6

Conductivity:

174 µS/cm (at 25°C, analysis: March 2017)

Nominal and measured concentrations:

nominal: 100 mg/L
measured: 0 h= 105.4 mg/L, 48 h old= 101.4 mg/L, 48 h new= 102.2 mg/L, 96 h= 90.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Type (delete if not applicable): open, covered with glass plate
- Material, size, headspace, fill volume: 12 L aquarium, fllled with 10 L tap water
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): after 48 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, Wasserwerk Kirrweiler
- Total organic carbon: 0.6 mg/L
- Metals: < 0.1 mg/L
- Pesticides: - Chlorine: <1 µg/L (chlorinated organic compounds)
- Intervals of water quality measurement: yearly

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod:12/12 hours using neon tubes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations were made every 24 hours, measuring pH and O2-concentration of the test solution in each vessel and documenting mortality

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: limit test
- Test concentrations: 100 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No abnormal behaviour of the surviving fish was observed.
- Mortality of control: No mortality occurred in the blank control.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: measured concentrations were in a range of 91 % and 105 % of the nominal concentration. Therefore, the determination of results was based on the nominal concentration.

Results with reference substance (positive control):

no positive control required

Reported statistics and error estimates:

no statistical evaluation was performed

Sublethal observations / clinical signs:

Table 2 Mortality

Nominal Conc. in mg/L	Total number of fish used	Number of dead fish
		24h	48h	72h	96h
Blank control	7	0	0	0	0
100	7	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

The study was performed as a limit test at the concentrations 100 mg/L. The concentration showed no mortality and no abnormal behaviour.

Executive summary:

An acute toxicity (96 h) assay on Danio rerio was conducted with the test item Hydroxyacetone, following OECD guideline 203 and EU-method C.1 and in compliance with GLP citreria. The study was performed as a limit test at the concentrations 100 mg/L in one valid experiment. To avoid volatilization of the test item, the study was performed in nearly complete filled aquaria, covered with a glass plate and without aeration. To ensure sufficient oxygen supply during the test, the study was performed under semi-static conditions and a medium renewal was performed after 48 hours. The content of the test item in the test solutions was determined using HPLC at the beginning and at the end of the test and at medium renewal in the old and the new solutions. The measured concentrations were in a range of 91 % and 105 % of the nominal concentration. Therefore, the determination of the results was based on the nominal concentration. The concentration showed no mortality, therefore, the NOEC (96 h) was >= 100 mg/L, LOEC (96 h) was > 100 mg/L and LC50 (96 h) was > 100 mg/L.

Description of key information

Danio rerio, 96 h, semi-static, OECD 203, GLP, limit test, LC50 > 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

An acute toxicity (96 h) assay on Danio rerio was conducted with the test item Hydroxyacetone, following OECD guideline 203 and EU-method C.1 and in compliance with GLP citreria. The study was performed as a limit test at the concentrations 100 mg/L in one valid experiment. To avoid volatilization of the test item, the study was performed in nearly complete filled aquaria, covered with a glass plate and without aeration. To ensure sufficient oxygen supply during the test, the study was performed under semi-static conditions and a medium renewal was performed after 48 hours. The content of the test item in the test solutions was determined using HPLC at the beginning and at the end of the test and at medium renewal in the old and the new solutions. The measured concentrations were in a range of 91 % and 105 % of the nominal concentration. Therefore, the determination of the results was based on the nominal concentration. The concentration showed no mortality, therefore, the NOEC (96 h) was >= 100 mg/L, LOEC (96 h) was > 100 mg/L and LC50 (96 h) was > 100 mg/L.