Registration Dossier
Registration Dossier
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EC number: 201-632-1 | CAS number: 85-79-0
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cinchocaine
- EC Number:
- 201-632-1
- EC Name:
- Cinchocaine
- Cas Number:
- 85-79-0
- Molecular formula:
- C20H29N3O2
- IUPAC Name:
- 2-butoxy-N-[2-(diethylamino)ethyl]quinoline-4-carboxamide
- Test material form:
- solid: particulate/powder
1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- Because the test item is poorly soluble in water, a test solution was prepared using a saturated solution method according to the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, OECD No. 23.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- semi-static
- Water media type:
- other: ISO medium
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 245 mg/L (as CaCO3)
- Test temperature:
- 20.2– 20.7°C
- pH:
- 7.60 – 8.32
- Dissolved oxygen:
- 7.7 – 8.9 mg/L
- Nominal and measured concentrations:
- The nominal concentrations of test item used in the main experiment were: 6.25, 12.5, 25, 50 and 100 % saturated solution. The corresponding measured geometric mean test item concentrations were: 3.44, 6.74, 13.27, 27.73 and 52.62 mg/L.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.69 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.03 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 27.73 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.74 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 13.27 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of BUTOXYAMIDE were the followings:
The 24h EC50 value: 17.69 mg/L (measured) (95 % conf. limits: 16.17 – 19.35 mg/L)
The 48h EC50 value: 14.03 mg/L (measured) (95 % conf. limits: 13.03 – 15.12 mg/L)
The 48h EC100 value: 27.73 mg/L (measured)
The 48h No-Observed Effect Concentration (NOEC): 6.74 mg/L (measured)
The 48h Lowest Observed Effect Concentration (LOEC): 13.27 mg/L (measured) - Executive summary:
The toxicity potential to aquatic invertebrates was performed according to OECD 202 guideline.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of BUTOXYAMIDE were the followings:
The 24h EC50 value: 17.69 mg/L (measured) (95 % conf. limits: 16.17 – 19.35 mg/L)
The 48h EC50 value: 14.03 mg/L (measured) (95 % conf. limits: 13.03 – 15.12 mg/L)
The 48h EC100 value: 27.73 mg/L (measured)
The 48h No-Observed Effect Concentration (NOEC): 6.74 mg/L (measured)
The 48h Lowest Observed Effect Concentration (LOEC): 13.27 mg/L (measured)
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