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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21.-25.08.17
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus
- Justification for species other than prescribed by test guideline: -
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD):
- Feeding during test
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
2.502 mmol/L
Test temperature:
20.1 – 22.7 °C
pH:
7.6 - 7.8
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
nominal: 100 mg/L
measured (geometric mean): 106 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Beakers, glass, nominal volume 50 mL.
- Type (delete if not applicable): -
- Material, size, headspace, fill volume: 20 ± 5 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter): -
- Renewal rate of test solution (frequency/flow rate): -
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): -
- Biomass loading rate: -

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7, CAS No 7778-50-9)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 106 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50 (24h) 1.6 mg/L
Validity criteria fulfilled:
yes
Conclusions:
All Daphnia exposed to the test item (100 mg/L) survived and only one Daphnia was immobilised in the blank control.Therefore, the EC50 (48 h) was determined to be > 106 mg/L.
Executive summary:

One valid experiment was performed.

The study was performed using one concentration at nominally 100 mg/L of test item and one control with four replicates each. 20 Daphnia were exposed to the test item (5 daphnia per replicate) for 48 hours in a static test system. After 23 and 48 hours, the immobilised Daphnia were counted.

All Daphnia exposed to the test item (100 mg/L) survived and only one Daphnia was immobilised in the blank control.

Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC.

The measured concentration of the test item at the beginning of the test was 85 % of the nominal concentration and at the end of the test 132 % of the nominal concentration.

Therefore, the determination of the biological results was based on the geometric mean of the measured concentration.


The following results were determined for the test item 2,2'-Azobis (4-methoxy-2,4-dimethyl valeronitrile) (species:Daphnia magna).

 

48h-NOEC≥106 mg/L
48h-LOEC > 106 mg/

24h EC 50 > 106 mg/L

48h EC 50 > 106 mg/L

Description of key information

No immobilisation is observed in the OECD 202 guideline study. The highest concentration is used as key value.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information