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EC number: 290-971-9 | CAS number: 90294-83-0
Assay : Bacterial mutation test using Salmonella typhimurium his-" and "Escherichia coli" WP(uvrA-)(pKM101) according to the OECD guideline n°471 (LEMI operating procedure n°MB08/45).
The test item has been tested for their capacity to induce revese mutation in four Salmonella typhimurium strains and one Escherichia coli WP2(uvrA-)(pKM101) strain. This study was performed in the absence and presence of metabolic activation. Two independent assays were carried out.
For assay n° 1, various concentrations were put in contact with the strains in the absence and presence of a metabolic activation system (S9 -mix 10% (v/v)).
For assay n°2, various concentration were put in contact with the strains in the absence of metabolic activation and with pre-incubation in the presence of metabolic activation system (S9 -mix 10% (v/v)) and without re-incubation for TA98 strain.
For the two assays, negative and positive controls were carried out in parallel. Positive controls induced a significant increase in the number of revertant colonies compared to negative controls. There is no significant difference between the number of spontaneous reversions, the number of reversions obtained in the positive controls (without and with metabolic activation), and the mean corresponding experimental "historical" values obtained in the laboratory.
These results validate the two tests.
There is an increase in the number of revertant colonies in Salmonella typhimurium TA 98:
- without metabolic activation in presence of 150, 500, 1500 and 5000 µg/plate
- with metabolic activation without and with pre-incubation in presence of 500, 1500 and 5000µg/plate
and in Salmonella typhimurium TA 100 without metabolic activation in presence of 5000 µg/plate.
The test item induce a mutagenic change in Salmonella typhimurium TA 98 and TA 100, according to the OECD Guideline n°471.
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